PTENP1
PTENP1, phosphatase and tensin homolog pseudogene 1 is a tumor suppressor gene and undergoes genomic deletion in human melanoma.
Contents
Annotated Information
Name
PTENP1: Phosphatase and tensin homolog pseudogene 1 (HGNC nomenclature) LINC00586[1]
Characteristics
PTENP1 is 3932 bp long non-coding RNA, located on human chromosome 9p13.3 (HGNC). PTENP1 is highly homologous to PTEN, with only 18 mismatches throughout the coding sequence [1].
Function
PTENP1 3'UTR can increase PTEN expression by binding to microRNAs that downregulate PTEN expression. Thus, PTENP1 plays an important role in cancer biology, restricting cell proliferation by acting as a microRNA decoy for the tumor suppressor PTEN [1].
The overexpressed PTENP1 repressed the oncogenic PI3K/AKT pathway and elicited pro-death autophagy via sequestering miR-17, miR19b and miR-20a in vitro, and inhibited the hepatocellular carcinoma (HCC) tumor growth in vivo, which was accompanied by enhanced apoptosis, autophagy and dampened angiogenesis/neovasculature maturation [3].
PTENP1 along with two other lncRNA CUDR, and LSINCT-5 was identified as a potential diagnostic marker for gastric cancer (GC) that distinguished between early-stage GC patients and healthy subjects [4].
Regulation
Histone acetylation is a key factor in controlling lncRNA BANCR expression [1].
Diseases
Expression
PTENP1 is downregulated in hepatocellular carcinoma (HCC) cells [3].
| Experiment | Forward primer | Reverse primer |
|---|---|---|
| PCR | 59-GAGGAGCCGTCAAATCCAGAG-39 | 59-TCGTCAATGTGTGAGGTTCC-39[1] |
| Real time PCR | 59-TCAGAACATGGCATACACCAA-39 | 59-TGATGACGTCCGATTTTTCA-39[1] |
Labs working on this lncRNA
- Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan.[3]
- Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei, Taiwan.[3]
- Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan.[3]
- Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, Taiwan.[3]
- Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, China.[4]
- Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.[4]
- Institute of Pathology, Fudan University, Shanghai, China.[4]
- Fudan University Shanghai Cancer Center-Institut MeRieux Laboratory, Fudan University Shanghai Cancer Center, Shanghai, China.[4]
- Institutes of Biomedical Sciences, Fudan University, Shanghai, China.[4]
References
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Poliseno L, Salmena L, Zhang J, Carver B, Haveman WJ & Pandolfi PP. A coding-independent function of gene and pseudogene mRNAs regulates tumor biology[J]. Nature. 2010, 465(7301):1033-1038.
- ↑ Martens-Uzunova ES, Bottcher R, Croce CM, Jenster G, Visakorpi T & Calin GA. Long noncoding RNA in prostate, bladder, and kidney cancer[J]. Eur Urol. 2014, 65(6):1140-1151.
- ↑ 3.0 3.1 3.2 3.3 3.4 3.5 3.6 Chen CL, Tseng YW, Wu JC, Chen GY, Lin KC, Hwang SM et al. Suppression of hepatocellular carcinoma by baculovirus-mediated expression of long non-coding RNA PTENP1 and MicroRNA regulation[J]. Biomaterials. 2015, 44:71-81.
- ↑ 4.0 4.1 4.2 4.3 4.4 4.5 4.6 Dong L, Qi P, Xu MD, Ni SJ, Huang D, Xu QH et al. Circulating CUDR, LSINCT-5 and PTENP1 long noncoding RNAs in sera distinguish patients with gastric cancer from healthy controls[J]. Int J Cancer. 2015, 137(5):1128-1135.
Sequence
>gi|11191|ref|NR_023917.1| Homo sapiens phosphatase and tensin homolog pseudogene 1 (PTENP1), long non-coding RNA <dnaseq>AGATGAGAGACAGAGACGGCGGCGGCGGCGGCGGCCCGGCCCGGCCCGGCCTGAAGCCCCTCTCAGCAAC CCGTGAGCATCCGCGGGGGCGGCGCCGGCAGCGGCGGCGTTTCTCGCTTCTTCTTCGTCTTTTCTAACTG TGCAGCCTCTTCCTCGGCTTCTCCTGAAAGGGAAGGTGGAAGCCGTGGGCTTGGGCGGGAGCCGGCTGAG GCGCGGCGGCGGCGGCACCTCCCCCTCCTGGAGCGGGGGGAGAAGCGGCGGCGGCGGCGCCCCCGGTGGC CCCGGCGGCTGCAGCTCCAGGGAGGGGGTCTGAGTCGCCTGTCACCATTTCCAGGGCTGGGAACGCCGGA GAGTTGGTCTCTCCCCTTCTACTGCCTCCAACACGGCGGCGGCGGCGGCGGCGGCGGCGGCACATGGAGG GACCCGGGCCGGTTTTAAACCTCTCGTCCGCCGCCGCCGCACCCCCCGTGGCCCGGGCTCCGGAGGCCGC TGGAGGAGGCAGCCGTTCGGAGGATTATTCGTCTTCTCCCCATTCCGCTGCCGCCGCTGCCAGGCCTCTG GCTGCTGAGGAGAAGCAGGCCCAGTAGCTGCCACCATCCAGCAGCTGCTGCCGCAGCCATTACCCGGCTG CGGTCCAGAGCCAAGCAGCGGCTGAGCGAGGGGCATCAGCTACCGCCAAGTCCAGAGCCATTTCCATCCT GCAGAGCCCCGCCACCAGCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAGACA GGACAGCCATCATCAAAGAGATCGTTAGCAGAAACAAAAGGAGATATCAAGAGGATGGATTCGACTTAGA CTTGACCTATATTTATCTAAACATTATTGCTATGGGATTTCCTGCAGAAAGACTTGAAGGCGTATACAGG AACAATATTGATGATGTAGTAAGGTTTTTGGATTCAAAGCATAAAAACCATTACAAGATACACAATCTTT GTGCTGAAAGACATTATGACACCGCCAAATCTAATTACAGAGTTGCGCAATATCCTTTTGAAGACCATAA CCCACCACAGCTAGAACTTATCAAACCCTTTTGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAAT CATGTTGCAGCAATTCACTGTAAAGCTGGAAAGGGACGAACTGGTATAATGATTTATGCATATTTATTAC ATCGGGGCAAATTTTTAAAGGCACAAGAGGCCCTAGATTTCTATGGGGAAGTAAGGACCAGAGACAAAAA GGGAGTAACTATTCCCAGTCAGAGGCGCTATGTGTATTACTATAGCTACCTGGTAAAGAATCATGTGGAT TATAGACCAGTGGCACTGTTGTTTCACAAGATGATGTTTGAAACTATTCCAATGTTCAGTGGCGGAACTT GCAATCCTCAGTTTGTGGTCTGCCAGCTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCCACACGATG GGAGGACAAGTTCATGTATTTTGAGTTCCCTCAGCCGTTACCTGTGTGTGGTGATATCAAAGTAGAGTTC TTCCACAAACAGAACAAGATGCTAAAAAAGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATAC CAGGACCAGAGGAAACCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATTGATAGCATTTG CAGTATAGAGCGTGCAGATAATGACAAGGAGTATCTAGTACTTACTTTAACAAAAAATGATCTTGACAAA GCAAATAAAGACAAAGCCAACCGATACTTTTCTCCAAATTTTAAGGTGAAGCTGTACTTCACAAAAACAG TAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACCAGATGTTAGTGACAATGAACC TGATCATTATAGATATTCTGACACCACTGACTCTGATCCAGAGAATGAACCTTTTGATGAAGATCAGCAT ACACAAATTACAAAAGTCTGAATTTTTTTTTATCAAGAGGGATAAAACACCATGAAAACAAACTTGAATA AACTGAAAAGGGACCTTTTTTTTTAATGGCAATAGGACATTGTGTCAGATTACCAGTTATAGAAACAATT CTCTTTTCGTGACCAATCTTGTTTTACCCTATACATCCACAGGGTTTTGACACTTGTTGTCCAGTTGAAA AAAGGTTGTGTAGCTGTGTCATGTATATACCTTTTTGTGTCAAAAGGACATTTAAAATTCAATTAGGATT AATAAAGATGGCACTTTCCCATTTTATTCCAGTTTTATAAAAAGTGGAGACAGACTGGTGTGTATACGTA GGAATTTTTTCCTTTTGTGTTCTGTCACCAACTGAAGTGGCTAAAGAGCTTTGTGATATACTGGTTCACA TCATACCCCTTTGCACTTGTGGCAACAGATAAGTTTGCAGTTAGCTAAGAGAAGTTTCTGAAGGGTTTTG CTGCATTCTTGCATGTATTTGGGTTAGGGGAATGGAGGGAATGCTCAGAAAGGAAATAATTTTATGCTGG ACTCTGGACCATATACCATCTCCAGCTATTTACACACACCTTTCTTTAGCATGCTACAGTTATTAATCTG GACATTCGAGGAATTGGCTGCTGTCACTGCTTGTTGTTTTCGCATTTTTTTAAAAGCATATTGGTGCTAG AAAAGGCAGCTAAAGGAAGTGAATCTGTATTGGGGTACAGGAATGAACCTTCTGCAACATCTTAAGATCC ACAAATGAAGGGATATAAAAATAATGTCATAGATAAGAAACACAGCAACAATGACTTAACCATATAAATG TGGAGGCTATCAACAAAGAATGGGCTTGAAACATTATAAAAATTGACAATGATTTGTTAAATATGTTTTC TTAATTGTAACGACTTCTCCATCTCCTGTGTAATCAAGGCCAGTGCTGAAAGTCAGATGCTATTAGTACC TACATCAGTCAACAACTTACACTTATTTTACTAGTTTTCAATCATATACCTGCTGTGAATGCTTCATGTG CTGCCTGCAAGCTTCTTTTTTCTCATTAAATATAAAATATTTTGTAATGCTAAAAAAAAAAAAAAGAACA TAGAAGACAAACATCAGAAATAAACCCATCCACTATGGTTAACTCATTTTTGAATATGGTGTAAAGTCAG TTTAATGATGAAATAATATTATTTTCAACAAATAGTACAGAAATTAAACTATGCAAAAATACTATTAATA ATGATGTTGGATCATTACCTCACACCATACCCCCAAATTAACTCAGAACATGGCATACACCAAATATAAG AGCAAAAACTATGAAATTTCTAGAAGAAGACAGAAAAAAAATTATGACTTTGGCTTAGTTGTTTCTTAGA AGTAACAGGAAAAGCATAATTCATAAAAGATAAATTGAAAAATCGGACGTCATCAAAATAAAAAATGGTT TATCTTCAAAGTCACCTGTTAAGAAAATGAGAAGACAAATCATAAAGCAGGGAGAAATTTTTGAAAGTCA TGTATCTGATAAGGGACAGTTAAAACTAAATAAAAACACAAACCATCCAATTAAAATGAATAAAGGGTTC GAATAGGTATGTCATCAGAGATCATATAGGAATAACCAATAAGCAAACAAAAGATGTTCAATATCATTAG TTATTAGAGAAACAAAATTTAAAAACCACAATGAGATGACACTACACATTTGCCAGAATGATGATTATTA AAAAAGACCAAGTATTGGAGAGGATGTGAAAAAACTGGAACCTCACACATTGACGATAGAAATGTAAAAT GAAATAGCCAATTCAGAAAACATTTTACAAGTTTTCTTTGTTTTTGTTTTTAATTTATTATACCCTTACC CTATGACTCAGTAATTCCACTTCTAGATACCTACCAAATAAAATTAAAGTTTATAACTTCACAAAGAAAA AAAAAAAAAAAA/dnaseq>
