LINC01672
LINC01672, long intergenic non-protein coding RNA 1672, an important key regulator of esophageal squamous cell carcinoma (ESCC) tumor metastasis and drug resistance.
Contents
Annotated Information
Name
LINC01672: long intergenic non-protein coding RNA 1672 (HGNC:52460)
Aliases
NMR: NSUN2 methylated lncRNA
Characteristics
5′ and 3′ RACE analyses were performed to determine the full length of NMR. 5′ and 3′ RACE analyses were performed to determine the full length of NMR. The full-length NMR was then validated by Northern blot. Northern blot identified strong bands around 1800 nt which was consistent with RACE results and NMR was overexpressed in tumor tissues. Cellular localization provides important information about potential functions of lncRNAs. RNA FISH and subcellular fractionation assays showed that most of NMR located in the cell nucleus rather than cytoplasm, red fluorescence which indicated NMR could only be seen in nucleus and over 95% of NMR was detected in nucleic RNAs. [1]
Function
Functionally, NMR could promote tumor cell migration and invasion, inhibit cisplatin-induced apoptosis and increase drug resistance in ESCC cells. NMR could directly bind to chromatin regulator BPTF, and potentially promote MMP3 and MMP10 expression by ERK1/2 pathway through recruiting BPTF to chromatin. Taken together, NMR functions as an oncogenic gene and may serve as new biomarker and therapeutic target in ESCC.[1]
Regulation
Transcription of NMR could be upregulated by NF-κB activation after IL-1β and TNF-α treatment. NMR was methylated by NSUN2 and might competitively inhibit methylation of potential mRNAs.[1]
Expression
LncRNA NMR is overexpressed in ESCC tissues. Lymph node positive HNSC patients had significantly higher NMR expression; meanwhile, overexpression of NMR was also significantly associated with distal tumor metastasis in head and neck squamous cell carcinoma (HNSC) patients. [1]
Diseases
Labs working on this lncRNA
- Department of Thoracic Surgery, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China
- Central Laboratory, Beijing Luhe Hospital, Capital Medical University, Beijing, China
- Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, Shanxi, 030001, China
- Tumor Hospital of Shanxi Province, Taiyuan, Shanxi, 030013, China
References
Sequence
>gi|100505887|ref|NR_104620.1| Homo sapiens long intergenic non-protein coding RNA 1672 (LINC01672), transcript variant 1, long non-coding RNA
000081 TGGCATGTGC ATGTAAATGT CAACAGATCA ATAAAGGCAA AAACCAGGAG ATCCCATAAA TCCCGCTGAG CCTGTCATAG 000160
000161 CTATCAAATT ATACCCAGAT AAAGCTTCTG GTGGCTAATG ACATGGCCAT CACCCAGCGT GGACGTGGGC AGGAGGGGCT 000240
000241 CTCCGGGAGC ATCTGGTCTG CTGTGGAGAC ACTTGTCATC TGGAGGCTCT GTGGCTCTAA TGGAGGGTGA TGCTTTGAAC 000320
000321 GCGACTATCT CCCCCATAAA AAAATAAATA AATAATAAGT ATAATTTAAA AATAAACAAA GATCTGGATT TGCAGGCTAA 000400
000401 AAAAAAAAAA A
