NONHSAT035954

AS1DHRS4, a head-to-head natural antisense transcript, silences the DHRS4gene cluster in cis and trans.

Annotated Information

Name

DHRS4-AS1:DHRS4 antisense RNA 1 (HGNC nomenclature)

Function

AS1DHRS4 Regulates DHRS4 Gene Cluster Transcription by interacting with H3K9 and H3K27 Methyltransferases.^[1]

AS1DHRS4 has both specific cis and trans activity on inducing different repressive enzyme complexes in the individual promoters within the DHRS4 gene cluster locus.^[1]

Expression

AS1DHRS4 is a cis-antisense transcript to the DHRS4 gene. ^[1]

Knockdown of AS1DHRS4 had no effect on DHRS2gene expression at the transcriptional level Knockdown of AS1DHRS4 increased both mature mRNAs and pre-mRNAs of the DHRS4 gene cluster^[1]

siRNA Name	Sequences
siASDhrs4-1	sense:AACUCAUUUGGUAAAAUGGTT^[1]
siASDhrs4-1	antisense:CCAUUUUACCAAAUGAGUUTT^[1]
siASDhrs4-2	sense:UGUUUCUGGAUGAAGACUCTTT^[1]
siASDhrs4-2	antisense:GAGUCUUCAUCCAGAAACATT^[1]

Labs working on this lncRNA

Department of Cell Biology, Shantou University Medical College, Shantou 515041, China

References

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Li Q, Su Z, Xu X, Liu G, Song X, Wang R, et al. AS1DHRS4, a head-to-head natural antisense transcript, silences the DHRS4 gene cluster in cis and trans[J]. Proceedings of the National Academy of Sciences of the United States of America. 2012,109(35):14110-5

Basic Information

Transcript ID	NONHSAT035954
Source	NONCODE4.0
Same with	DHRS4-AS1,C14orf167, "chromosome 14 open reading frame 167", "DHRS4 antisense RNA 1 (non-protein coding)"
Classification	antisense
Length	3038 nt
Genomic location	chr14-:24407940..24423392
Exon number	2
Exons	24407940..24410367,24422783..24423392
Genome context
Sequence	000001 AGAACACTGG GGAGACTAGA TCGATAGGGC TGCACAGAGG TGGCACAGTT GTGCCAGAGG TAGCAGGGAC AGGAGGGCTG 000080 000081 GCAAGGGAGG GTCCAGGGCT AGGGGATTCG GAGTGGCTTC AGGCGGACCA CGTGGCTACC TTTTTTCCAG GGCCAGACTT 000160 000161 TGGCAGCGTG TAAGGTCTGA GGACAGGGGC ACCGGAGGCC GAGGATGAGA GGCCAGTGCC TGTTTCCAGG CAGCCAGGGC 000240 000241 CTCAGAAACT CCGGCCGGAG CACTCACCCG TCGGTGGAGG CCGTTACCAG GGCCACCTTA TTTGCGAGCG GGTCCCGGCG 000320 000321 GGTCATCCCG GAGCTGGCCA TCCGCACCGA ATTCCAAGCC CGGGCACAGA GGCCTAGCAG CCCCGCCTTG TGCATGGATC 000400 000401 AGACCAGCAA GTATGGGTTC CACTCTTCCC AGGGGCGGTG ACAGAGTAGG GCGAGGGGCA GGACGAAGGG CGGGCCGATT 000480 000481 CCCTGCCCGC CCTGCTCCCC GCCCCGGTAC CGCTGTGAGC GGTCTGTGGC CCTGGGCACC TCCATCCGCC GCGGCGCCCC 000560 000561 TCCACCATCT ACCTTCCGCC TGACTGGGGC TTGCCTTTCC GGGCTGGCGG GTGCCACTTC GGATAAACCC TTTGGACTCC 000640 000641 TAACTCCAAT CAGGTGTCTG CTTTGTTGAG GACTCACAGA CACAGTCTCC TTTCTTCAAG ATCTTTACAA TGCAAGACCT 000720 000721 CACTAACACA CAGGGATGGT CTCCCAGAGG GTCTGTGCTG TTCCTTCACT CAGAACATCA AGATGCACTG AAGTAAGGAT 000800 000801 CCTCTATTCT ACAGTTCCTG CTAGCTGAGC TATTCCATGG GGGCTTCAGC AGGAAATTCC AAGGTTGGCT TTGACAAGCT 000880 000881 AAGGCCGGCT GGTGGAGCAC ATCGAGTTCT GGAGGTTCAT GTGTGTTTTC ATGAAGATCT GTCTGCCCGT AGCAGATAAA 000960 000961 GAGTTGTTGC CCCACTCCTC CTGGGGTCTT CTATTTTCCT GGGAGGAATT TCTGGATTAA CTGAACACAC ACACACACAC 001040 001041 ACACACCCTT TTGAAGCATC AACAGTAATT CTGAGTTCTT AGGGACAATG CAGATTAAAT CCACAATAAG AAAGACAACT 001120 001121 ATGGCCAGGT GTGGTGGCTC ACGCCTGTAA TCCCAGAACT TTGGGAGGCT GAGGCGGATG GATCACCTGA GGTCAGGAGT 001200 001201 TAGAGACCAA CCTGACCAAC ATGGAGAAAC CCCGTTTCTA CTAAAAATGC AAAATTAGCC GGGCATGGTG GCAGGCGCCT 001280 001281 GTAATCCCAA ATACTCGGGA GGCTGAGGCA GGAGAATCAC TTAAACCCGG GAGGCAGAGG TTGCAGTGAG CCAAGATCGC 001360 001361 GCCATTGCAC TCCAGCCTGG GCAAGAAGAG CAAAACTCCA TCTCAAAAAA AAAAAAAGAG TACTATTGAT CCAGAGGTGA 001440 001441 CCAGACTAGC CTCACTGGAG ATAATAGGTG ACATTATTAT GCAAAGTGGT TTCAGTTCCC TTGTCTTGTT TTTGGAATAG 001520 001521 TATAAGAGGC CTACAATACG AATAAATAAG GCCCATCACA AGCCTCACCA AAGGACAAAA CTAATCCACT TCTGAAGAAG 001600 001601 GCTCTTTGTT GGGCCAGATC AAATCCTTGG ACAGAACTGT ACTCTTAATC TCTGTAGTGA AAGTGGGGGG TAGTTTAAGA 001680 001681 ATTTATTAAC CTTCTACCTT GAGCAATCCC TCAGAAGTAA GAGGTGTCAG GCCTCTGAGC CGAAGCTCAG CCATTGTAAC 001760 001761 CCCTGTGACC TGCACATATA TGTCCAGATG GCCTGCAGGA GCCAAGAAGT CTGGGGCAGC TGAAAAACCA CACAAGAAGT 001840 001841 AAAACAGCCA GTTCCTGCCT TAACTGATTA ACCAACATTA CAACATTCCA CCATTGTGAC CTGTCCCTGT CCTACCTTAA 001920 001921 CTGATCAATC GACCTTGTGA CAGTCTTCTT CTGGACAATG GGTCTTATCA TCTCCCCACC ATGTACCTTG TGACCCCCTC 002000 002001 CTCTGCTAAC AATAGATAAC CACTTTTTAC TGTAATTTTC CATTACCTAC CCAACTCCTA TAAAGCAACC CCTTCCCCAT 002080 002081 CTCCCTTCAC TGACTCTGTT TTCAGACTCA GCCCACTTGC ACTCAAGTGA ATAAACAGCC TTGTTGCTCA CACAAAGCC

[ref1-1] 1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Li Q, Su Z, Xu X, Liu G, Song X, Wang R, et al. AS1DHRS4, a head-to-head natural antisense transcript, silences the DHRS4 gene cluster in cis and trans[J]. Proceedings of the National Academy of Sciences of the United States of America. 2012,109(35):14110-5

[1]