ENST00000444200.1

APOA1-AS, an antisense transcript of APOA1, functions as a negative transcpritional regulator of APOA1 by modulating distinct histone methylation patterns.

Annotated Information

Name

Figure 1. APO gene cluster and APOA1-AS NAT organization on human chromosome 11. .

APOA1-AS: Apolipoprotein A-1 antisense RNA(HGNC nomenclature)

Characteristics

APOA1-AS is transcribed from the positive strand of chromosome 11 and has two exons, positioned ~20kb apart. It shares a 123 nucleotide-long region overlapping with the fourth exon of APOA1 mRNA.(Figure 1)

Function

Figure 2. Characterization of the expression profiles of APOA1 and APOA1-AS. .

APOA1-AS functions in cis to modulate histone methylation patterns of APOA1, through the recruitment of LSD1 and PRC2. Targeting APOA1-AS using short antisense oligonucleotides will enhance APOA1's expressopn level. APOA1-AS also regulates the transcription of APOC3 and APOA4, which are located in close proximity on the chromatin.

Expression

APOA1 and APOA1-AS are expressed in all tissues examined, but there their expression levels are quite different. APOA1 mRNA’s expression level is highest in liver, small intestine and colon, while APOA1-AS is highly expressed in ovary, cervix, testis and thyroid. The ratio of APOA1/APOA1-AS varies among the tissues examined.(Figure 2)

SiRNA name	Sequence
APOA1-AS1	5'-CCAAATGGCAAACCTTCTT-3'
APOA1-AS2	5'-GGCTACTTATTGCTGGAAA-3'
APOA1-AS3	5'-CCTTGGATTGAAGTAATC-3'

Labs working on this lncRNA

Center for Therapeutic Innovation & Department of Psychiatry and Behavioral Sciences, University of Miami Miller School of Medicine .

Basic Information

Transcript ID	ENST00000444200.1
Source	Gencode19
Same with	lnc-APOC3-1:1,NONHSAT024360
Classification	antisense
Length	563 nt
Genomic location	chr11+:116706833..116726445
Exon number	2
Exons	116706833..116706955,116726006..116726445
Genome context
Sequence	000001 CAGCTTCTCT TGCAGCTCGT GCAGCTTCTG GCGCGCGCCC TCTTGGAGCT CTGCGCGCAG CGGCTCCACC TTCTGGCGGT 000080 000081 AGAGCTCCAT CTCCTCCTGC CACTTCTTCT GGAAGTCGTC CAGATCCAAA TGGCAAACCT TCTTCATCCA CCAGGACCCA 000160 000161 ACCCACAGGC TACTTATTGC TGGAAACCTA CGTTGTTCCT TGGATTGAAG TAATCTCTCC CTCCTCTGGT GCGCCCACAG 000240 000241 CACTTGCACC AACAGTGGGT ACTCAACAGA CTAGCGTGCC TGCCGAAGAA GGGGTCCTCT GACAATCAGG GGACAATGGG 000320 000321 GAATTATGCT CTCCAGACTT TCTACACACA CAAGTCACAC AGGAAGGAAG GTAAAGAGAA ACTAGAGAAA ATAATTTTTG 000400 000401 AAGAAAAACA TTTCAGGAAG TATTGAAAGT ACACGGTAAC TCAGCCTGGG GCAGGGGTGG AGGGCAGCAG CACTGTTTGC 000480 000481 TGCAGCTATG CTCCTTCCTC AGTGCCCTGC ACACCCGGGA CTTGCTCGGT GAGCATCTCT CGTGTCAGTG ACAGCTAGTG 000560 000561 TGA

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