Difference between revisions of "NONHSAT056039"

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SNHG16,shorted forsmall nucleolar RNA host gene 16, which is located on 17q25.1 and has two splice variants, palys significant roles in cancers.
+
SNHG16, small nucleolar RNA host gene 16, serve as an oncogene that promoted tumor progression by acting as an endogenous 'sponge' to regulate microRNA.
 
==Annotated Information==
 
==Annotated Information==
===Transcriptomic Nomeclature===
+
===Name===
''SNHG16'', small nucleolar RNA host gene 16(HGNC nomenclature)
+
''SNHG16'', small nucleolar RNA host gene 16 (HGNC nomenclature), ''ncRAN'', "non-coding RNA expressed in aggressive neuroblastoma"<ref name="ref1" />
 
 
''ncRAN'', "non-coding RNA expressed in aggressive neuroblastoma"<ref name="ref1" />
 
  
 
===Characteristics===
 
===Characteristics===
ncRAN, non-coding RNA expressed in aggressive neuroblastoma (ncRAN), is located on 17q25.1 and has two splice variants, a long form (Nbla10727) and a short form (Nbla12061)<ref name="ref2" />
+
''SNHG16'' is a four exon transcript of  2538 bp (GenBank) long non-coding RNA, located on 17q25.1 and has two splice variants, a long form (Nbla10727) and a short form (Nbla12061) <ref name="ref2" />.  Non-primate metazoan species, such as mouse, rat, dog, cow, horse, zebrafish, or C. elegans, do not have ''SNHG16'' in their genomes <ref name="ref1" />.
  
 
===Function===
 
===Function===
ncRAN may be a novel non-coding RNA mapped to the region of 17q gain and act like an oncogene in aggressive neuroblastomas<ref name="ref1" />.
+
''ncRAN'' may be a novel non-coding RNA mapped to the region of 17q gain and act like an oncogene in aggressive neuroblastomas,glioma and cervical tumerigeneis <ref name="ref1" /><ref name="ref4" /><ref name="ref5" />.
 +
 
 +
''ncRAN'' may play an important role in the pathogenesis of human bladder cancer, and may help in designing effective therapy targeting the ncRAN pathway to control bladder cancer growth and invasion<ref name="ref2" />
 +
 
 +
''ncRAN'' might represent a novel prognostic indicator, a biomarker for the early detection of metastasis and a target for gene therapy in CRC <ref name="ref3" />.
  
ncRAN may play an important role in the pathogenesis of human bladder cancer, and may help in designing effective therapy targeting the ncRAN pathway to control bladder cancer growth and invasion<ref name="ref2" />
+
''SNHG16'' promotes glioma tumorigenesis by sponging miR-20a-5p, leading to the enhancement of its endogenous targets ''E2F1'' <ref name="ref4" />.
  
ncRAN might represent a novel prognostic indicator, a biomarker for the early detection of metastasis and a target for gene therapy in CRC<ref name="ref3" />.
+
''SNHG16''  promoted cervical tumor progression by acting as an endogenous ‘sponge’ to regulate miR-216A-5p/ZEB1 <ref name="ref5" />.
  
 
===Expression===
 
===Expression===
ncRAN expression is dramatically up-regulated and associated with poor prognosis in human neuroblastoma<ref name="ref1" />.
+
''ncRAN'' expression is dramatically up-regulated and associated with poor prognosis in human neuroblastoma <ref name="ref1" />.
 +
 
 +
''SNHG16'' is upregulated in cervical cancer tissues and cell lines <ref name="ref5" />.  
  
 
The primers of RT-PCR were as follows,
 
The primers of RT-PCR were as follows,
Line 35: Line 39:
 
|}
 
|}
  
 +
===Disease===
 +
* Bladder cancer <ref name="ref2" />
 +
* Cervical cancer <ref name="ref5" />
 +
* Colorectal cancer <ref name="ref3" />
 +
* Glioma <ref name="ref4" />
 +
* Neuroblastoma <ref name="ref1" />
  
 
==Labs working on this lncRNA==
 
==Labs working on this lncRNA==
*Division of Biochemistry and Innovative Cancer Therapeutics, Chiba Cancer Center Research Institute, Chiba 260-8717, Japan<ref name="ref1" />
+
* Division of Biochemistry and Innovative Cancer Therapeutics, Chiba Cancer Center Research Institute, Chiba 260-8717, Japan.<ref name="ref1" />
.
+
 
* Department of Urology, the First affiliated Hospital of China Medical University, China<ref name="ref2" />.
+
* Department of Urology, the First affiliated Hospital of China Medical University, China.<ref name="ref2" />
 +
 
 +
* Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China; Institute of Pathology, Fudan University, Shanghai, China.<ref name="ref3" />
  
* Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China; Institute of Pathology, Fudan University, Shanghai, China<ref name="ref3" />.
+
* Department of Obstetrics and Gynecology, Xin Hua Hospital, Shanghai Jiao Tong University School of Medicine, No.1665, KongJiang Road, YangPu District, Shanghai 200092, China.<ref name="ref5" />
  
 
==References==
 
==References==
 
<references>
 
<references>
<ref name="ref1">Yu M, Ohira M, Li Y, Niizuma H, Oo ML, Zhu Y, et al. High expression of ncRAN, a novel non-coding RNA mapped to chromosome 17q25.1, is associated with poor prognosis in neuroblastoma[J]. International journal of oncology. 2009,34(4):931-8.</ref>(1)
+
<ref name="ref1"> Yu M, Ohira M, Li Y, Niizuma H, Oo ML, Zhu Y, et al. High expression of ncRAN, a novel non-coding RNA mapped to chromosome 17q25.1, is associated with poor prognosis in neuroblastoma[J]. International journal of oncology. 2009,34(4):931-8.</ref>(1)
<ref name="ref2">Zhu Y, Yu M, Li Z, Kong C, Bi J, Li J, et al. ncRAN, a newly identified long noncoding RNA, enhances human bladder tumor growth, invasion, and survival[J]. Urology. 2011,77(2):510 e1-5.</ref>(2)
+
<ref name="ref2"> Zhu Y, Yu M, Li Z, Kong C, Bi J, Li J, et al. ncRAN, a newly identified long noncoding RNA, enhances human bladder tumor growth, invasion, and survival[J]. Urology. 2011,77(2):510 e1-5.</ref>(2)
<ref name="ref3">Qi P, Xu MD, Ni SJ, Shen XH, Wei P, Huang D, et al. Down-regulation of ncRAN, a long non-coding RNA, contributes to colorectal cancer cell migration and invasion and predicts poor overall survival for colorectal cancer patients[J]. Molecular carcinogenesis. 2014.</ref>(3)
+
<ref name="ref3"> Qi P, Xu MD, Ni SJ, Shen XH, Wei P, Huang D, et al. Down-regulation of ncRAN, a long non-coding RNA, contributes to colorectal cancer cell migration and invasion and predicts poor overall survival for colorectal cancer patients[J]. Molecular carcinogenesis. 2014.</ref>(3)
 +
<ref name="ref4"> Yang BY, Meng Q, Sun Y, Gao L & Yang JX. Long Non-Coding Rna Snhg16 Contributes to Glioma Malignancy by Competitively Binding Mir-20a-5p with E2f1[J]. J Biol Reg Homeos Ag. 2018, 32(2):251-261.</ref>(4)
 +
<ref name="ref5"> Zhu H, Zeng Y, Zhou CC & Ye WP. SNHG16/miR-216-5p/ZEB1 signal pathway contributes to the tumorigenesis of cervical cancer cells[J]. Arch Biochem Biophys. 2018, 637:1-8.</ref>(5)
 
</references>
 
</references>
  
{{basic|
+
===Sequence===  
tID = NONHSAT056039|
+
>gi|100507246|ref|NR_038108.1| Homo sapiens small nucleolar RNA host gene 16 (SNHG16), transcript variant 1, long non-coding RNA
source = NONCODE4.0|
+
<dnaseq>GCGTTCTTTTCGAGGTCGGCCGCGTGGCTGGAAGACATGGCCACTCCAGTCGGTGTTGAGCACGGCGAGC
same = ''SNHG16'', ''ncRAN''|
+
AGTCTCAGGCCTTTAGTGATGATGATTAAAGGTGGTGGCTGTGGCCTTGAAAACAGTCATGTGAAAACTC
classification = intergenic|
+
ATCACCTTAAGGTGTTAAGTGTAAGGATCTTCATGATGAAATTTCTGTAAATGGTGCAGTCAGCCTCAGT
length = 2538 nt|
+
TTCCAAAGCCGGAAAAGGATCCTCTAGTAGCCACGGTGTGGCAGCTGCTCTGAACCAGGACCTGGACCCG
location = chr17+:74553846..74561430|
+
GACCCAAAGTGCCATGTCTTTAATGTGAGTTAGCTCCCAGCGATGCCAGATGGGATCAGCACAGCCCTGC
number = 4|
+
CTCTGCTGCTAATTGTTCCTCTAAAGTAATCGCCATGCGTTCTTTGGGCTTCATCTTTAAAGGAATGAAG
exons = 74553846..74553939,74555027..74555125,74557370..74557484,74559201..74561430|
+
CAACTGAGATTATTCTGGAAAACCTTTTGGCAGTTAGTGAAATTAGAGTACAACTAAGAACATTTTCAGA
context = <html><div align="center">
+
CCTCCACTGTGGATGACCTGGGTATAATCTCACAAATCGATGGGACTGCAAGGATTGTAAACTGAAATGA
<iframe src="http://lncrna.big.ac.cn/view/?data=species/human&loc=chr17:74553846..74561430&tracklist=0&overview=0&tracks=DNA,RefGene,lncRNA" style=" border-width:0 " width="100%" height="250" scrolling="yes"></iframe>
+
ACATGATTATACTCTGTTGGAAGAGCCTAAGAGGAAACTGATGCCATGAGTTTCAGAGAGTAATGCTTAA
</div></html>|
+
CCCCAGTTACACAGGATGCCGTCTTGTGTTTCCTCTTGTTTAGTTACCCACTACAGTGATTTTGTGATCT
sequence = <dnaseq>GCGTTCTTTTCGAGGTCGGCCGCGTGGCTGGAAGACATGGCCACTCCAGTCGGTGTTGAGCACGGCGAGCAGTCTCAGGCCTTTAGTGATGATGATTAAAGGTGGTGGCTGTGGCCTTGAAAACAGTCATGTGAAAACTCATCACCTTAAGGTGTTAAGTGTAAGGATCTTCATGATGAAATTTCTGTAAATGGTGCAGTCAGCCTCAGTTTCCAAAGCCGGAAAAGGATCCTCTAGTAGCCACGGTGTGGCAGCTGCTCTGAACCAGGACCTGGACCCGGACCCAAAGTGCCATGTCTTTAATGTGAGTTAGCTCCCAGCGATGCCAGATGGGATCAGCACAGCCCTGCCTCTGCTGCTAATTGTTCCTCTAAAGTAATCGCCATGCGTTCTTTGGGCTTCATCTTTAAAGGAATGAAGCAACTGAGATTATTCTGGAAAACCTTTTGGCAGTTAGTGAAATTAGAGTACAACTAAGAACATTTTCAGACCTCCACTGTGGATGACCTGGGTATAATCTCACAAATCGATGGGACTGCAAGGATTGTAAACTGAAATGAACATGATTATACTCTGTTGGAAGAGCCTAAGAGGAAACTGATGCCATGAGTTTCAGAGAGTAATGCTTAACCCCAGTTACACAGGATGCCGTCTTGTGTTTCCTCTTGTTTAgttacccactacagtgattttgtgatctgctaatgggttgccacccacaACCATTGCTTTAGCACTTTTACTTCAAATCAATGAAGGATTGATAAAAGTTCTCCTGGTGTCTCCGCAGAGTGCCTTCCAGGAACAGATCTTTGCATAGAATATCAGTGGTTTCCTTTTTTGTTTCAAATAGTGGTCAGAAAATACCCAGTGTTGACTCACCAAGGCAATCAGCTTCCTTTTTCCCtttttttgttttttttttaacattttatatttttgctttattttattttattttattttattttattttattttattttattttttgagacggagttccactctgtcgccaggctggagtgaagtggtacaatcttggctcactgcaacctccacctcccgggttcaagcaattctcctggctcagcctcctgagtgctgggactacaggcgcgtaccttctttagtagagactgggtttcaccatgttggccaggatggtctctatctcctgaccttgtgatctgcctgcctcagcttcccaaagtgctgagatgacaggtgtgagccatcagacccagcatttttttttttaatttaaatttaaattttttttcatttttttgagaggttttttttgttttgttttgttgttgttgttgttgttgtttttgagacagtcttgctctgtcacccaggctgggagtgcagtggcatgatctctgcaacctctacctcccaggttcaagcaattcttgtgcctcagcctcccaagtaactgggactacaggtgcacgctaccacacctggctgattttttttgttttagtagagacagggtttcaaccatgttgcccaggttggtctcaaactcctgagctcaggcaatccacccgccttggcctcccaaagtgctaggattacaggtgtgagccaccacacccagcTAttttttctttcgttttttaattttaaagttgggggggggtctcaatttgttaccctggctggtctcgaactcccggacttaagcgatcctcTGGCTCCAAGCCCACTACCAGTCTCAGGTTTCTTTACTAAAAGATCACTACCTTTTTTtctcttatctgctgccatgtgagatgtggctttcaccttccgccatgattgtgaggccttcccagccatgtagaactgtaagtccaataaacctcttttgtaaattaAAAAAAAAAAATCACTATTTAAGATACTAGGATGGATTGTGACTGTTGAGGAGTACTTACATATCCTACATTTGACTACATTATTTCCAAACCAAGTATTCCATCCAAAGGAACATACTGCTATCATAGAGACCAAGGAGGGACTGTTTAAGGTTGCCAAGGTGAAGCGAGCTGAGAGGCTTTGTCCTCGTGCCAGTAACTCTGAAATCTCTCTTAATTCCTGCTGTCCAGGCAGCAGAATGCCATGGTTTCCCCAAGTAGGTAGCTGCTTTAGCAGTTAAAGCCCAAATGTCTGTTCTGTTGATCAGAGGTCTCTGAATTTCTGAAGTGGTGTTTCGTTTCTGGTGACTGAGTTAATCCTTTACAATCCCTCTTGTAAAGTGTGCTAATAGAAAGAATCCACCTTTCAAAGCTGCAGAACCAGACCGTGCCCTAAATTGACCAACGTAACTGATGTGCCTCAGGAAGTCTCTTGCCAGCTGTCCCTGTGAAGACCCCCCTCCTCCCCCCCAGCTGCTGCCTTGCACACTGAAGCATCTCAGACTGTGCAAAGCCGTGTAGTCATCAAGACAGTAAATCCCAGGGCTTGGTTAAGTGCTGTGTGATAACTTGTTTGGATGAGACTTAACTTAAAACCACTTACAATAAACTTGGGAAACTACCGTCA</dnaseq>|
+
GCTAATGGGTTGCCACCCACAACCATTGCTTTAGCACTTTTACTTCAAATCAATGAAGGATTGATAAAAG
}}
+
TTCTCCTGGTGTCTCCGCAGAGTGCCTTCCAGGAACAGATCTTTGCATAGAATATCAGTGGTTTCCTTTT
[[Category:Intergenic]][[Category:NONHSAG022825]][[Category:Transcripts]]
+
TTGTTTCAAATAGTGGTCAGAAAATACCCAGTGTTGACTCACCAAGGCAATCAGCTTCCTTTTTCCCTTT
 +
TTTTGTTTTTTTTTTAACATTTTATATTTTTGCTTTATTTTATTTTATTTTATTTTATTTTATTTTATTT
 +
TATTTTATTTTTTGAGACGGAGTTCCACTCTGTCGCCAGGCTGGAGTGAAGTGGTACAATCTTGGCTCAC
 +
TGCAACCTCCACCTCCCGGGTTCAAGCAATTCTCCTGGCTCAGCCTCCTGAGTGCTGGGACTACAGGCGC
 +
GTACCTTCTTTAGTAGAGACTGGGTTTCACCATGTTGGCCAGGATGGTCTCTATCTCCTGACCTTGTGAT
 +
CTGCCTGCCTCAGCTTCCCAAAGTGCTGAGATGACAGGTGTGAGCCATCAGACCCAGCATTTTTTTTTTT
 +
AATTTAAATTTAAATTTTTTTTCATTTTTTTGAGAGGTTTTTTTTGTTTTGTTTTGTTGTTGTTGTTGTT
 +
GTTGTTTTTGAGACAGTCTTGCTCTGTCACCCAGGCTGGGAGTGCAGTGGCATGATCTCTGCAACCTCTA
 +
CCTCCCAGGTTCAAGCAATTCTTGTGCCTCAGCCTCCCAAGTAACTGGGACTACAGGTGCACGCTACCAC
 +
ACCTGGCTGATTTTTTTTGTTTTAGTAGAGACAGGGTTTCAACCATGTTGCCCAGGTTGGTCTCAAACTC
 +
CTGAGCTCAGGCAATCCACCCGCCTTGGCCTCCCAAAGTGCTAGGATTACAGGTGTGAGCCACCACACCC
 +
AGCTATTTTTTCTTTCGTTTTTTAATTTTAAAGTTGGGGGGGGGTCTCAATTTGTTACCCTGGCTGGTCT
 +
CGAACTCCCGGACTTAAGCGATCCTCTGGCTCCAAGCCCACTACCAGTCTCAGGTTTCTTTACTAAAAGA
 +
TCACTACCTTTTTTTCTCTTATCTGCTGCCATGTGAGATGTGGCTTTCACCTTCCGCCATGATTGTGAGG
 +
CCTTCCCAGCCATGTAGAACTGTAAGTCCAATAAACCTCTTTTGTAAATTAAAAAAAAAAAATCACTATT
 +
TAAGATACTAGGATGGATTGTGACTGTTGAGGAGTACTTACATATCCTACATTTGACTACATTATTTCCA
 +
AACCAAGTATTCCATCCAAAGGAACATACTGCTATCATAGAGACCAAGGAGGGACTGTTTAAGGTTGCCA
 +
AGGTGAAGCGAGCTGAGAGGCTTTGTCCTCGTGCCAGTAACTCTGAAATCTCTCTTAATTCCTGCTGTCC
 +
AGGCAGCAGAATGCCATGGTTTCCCCAAGTAGGTAGCTGCTTTAGCAGTTAAAGCCCAAATGTCTGTTCT
 +
GTTGATCAGAGGTCTCTGAATTTCTGAAGTGGTGTTTCGTTTCTGGTGACTGAGTTAATCCTTTACAATC
 +
CCTCTTGTAAAGTGTGCTAATAGAAAGAATCCACCTTTCAAAGCTGCAGAACCAGACCGTGCCCTAAATT
 +
GACCAACGTAACTGATGTGCCTCAGGAAGTCTCTTGCCAGCTGTCCCTGTGAAGACCCCCCTCCTCCCCC
 +
CCAGCTGCTGCCTTGCACACTGAAGCATCTCAGACTGTGCAAAGCCGTGTAGTCATCAAGACAGTAAATC
 +
CCAGGGCTTGGTTAAGTGCTGTGTGATAACTTGTTTGGATGAGACTTAACTTAAAACCACTTACAATAAA
 +
CTTGGGAAACTACCGTCA</dnaseq>

Latest revision as of 02:35, 19 November 2018

SNHG16, small nucleolar RNA host gene 16, serve as an oncogene that promoted tumor progression by acting as an endogenous 'sponge' to regulate microRNA.

Annotated Information

Name

SNHG16, small nucleolar RNA host gene 16 (HGNC nomenclature), ncRAN, "non-coding RNA expressed in aggressive neuroblastoma"[1]

Characteristics

SNHG16 is a four exon transcript of 2538 bp (GenBank) long non-coding RNA, located on 17q25.1 and has two splice variants, a long form (Nbla10727) and a short form (Nbla12061) [2]. Non-primate metazoan species, such as mouse, rat, dog, cow, horse, zebrafish, or C. elegans, do not have SNHG16 in their genomes [1].

Function

ncRAN may be a novel non-coding RNA mapped to the region of 17q gain and act like an oncogene in aggressive neuroblastomas,glioma and cervical tumerigeneis [1][3][4].

ncRAN may play an important role in the pathogenesis of human bladder cancer, and may help in designing effective therapy targeting the ncRAN pathway to control bladder cancer growth and invasion[2]

ncRAN might represent a novel prognostic indicator, a biomarker for the early detection of metastasis and a target for gene therapy in CRC [5].

SNHG16 promotes glioma tumorigenesis by sponging miR-20a-5p, leading to the enhancement of its endogenous targets E2F1 [3].

SNHG16 promoted cervical tumor progression by acting as an endogenous ‘sponge’ to regulate miR-216A-5p/ZEB1 [4].

Expression

ncRAN expression is dramatically up-regulated and associated with poor prognosis in human neuroblastoma [1].

SNHG16 is upregulated in cervical cancer tissues and cell lines [4].

The primers of RT-PCR were as follows,

primer Forward Reverse
ncRAN(long-form) 5'-CAGTCAGCCTCAGTTTCCAA-3' 5'-AGGCAGGGCTGTGCTGAT-3'[2]
ncRAN (short-form) 5'-ATGTTAGCTCCCAGCGATGC-3' 5'-CTAACTGCCAAAAGGTTTTCC-3'[2]

Disease

  • Bladder cancer [2]
  • Cervical cancer [4]
  • Colorectal cancer [5]
  • Glioma [3]
  • Neuroblastoma [1]

Labs working on this lncRNA

  • Division of Biochemistry and Innovative Cancer Therapeutics, Chiba Cancer Center Research Institute, Chiba 260-8717, Japan.[1]
  • Department of Urology, the First affiliated Hospital of China Medical University, China.[2]
  • Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China; Institute of Pathology, Fudan University, Shanghai, China.[5]
  • Department of Obstetrics and Gynecology, Xin Hua Hospital, Shanghai Jiao Tong University School of Medicine, No.1665, KongJiang Road, YangPu District, Shanghai 200092, China.[4]

References

  1. 1.0 1.1 1.2 1.3 1.4 1.5 Yu M, Ohira M, Li Y, Niizuma H, Oo ML, Zhu Y, et al. High expression of ncRAN, a novel non-coding RNA mapped to chromosome 17q25.1, is associated with poor prognosis in neuroblastoma[J]. International journal of oncology. 2009,34(4):931-8.
  2. 2.0 2.1 2.2 2.3 2.4 2.5 Zhu Y, Yu M, Li Z, Kong C, Bi J, Li J, et al. ncRAN, a newly identified long noncoding RNA, enhances human bladder tumor growth, invasion, and survival[J]. Urology. 2011,77(2):510 e1-5.
  3. 3.0 3.1 3.2 Yang BY, Meng Q, Sun Y, Gao L & Yang JX. Long Non-Coding Rna Snhg16 Contributes to Glioma Malignancy by Competitively Binding Mir-20a-5p with E2f1[J]. J Biol Reg Homeos Ag. 2018, 32(2):251-261.
  4. 4.0 4.1 4.2 4.3 4.4 Zhu H, Zeng Y, Zhou CC & Ye WP. SNHG16/miR-216-5p/ZEB1 signal pathway contributes to the tumorigenesis of cervical cancer cells[J]. Arch Biochem Biophys. 2018, 637:1-8.
  5. 5.0 5.1 5.2 Qi P, Xu MD, Ni SJ, Shen XH, Wei P, Huang D, et al. Down-regulation of ncRAN, a long non-coding RNA, contributes to colorectal cancer cell migration and invasion and predicts poor overall survival for colorectal cancer patients[J]. Molecular carcinogenesis. 2014.

Sequence

>gi|100507246|ref|NR_038108.1| Homo sapiens small nucleolar RNA host gene 16 (SNHG16), transcript variant 1, long non-coding RNA

000001 GCGTTCTTTT CGAGGTCGGC CGCGTGGCTG GAAGACATGG CCACTCCAGT CGGTGTTGAG CACGGCGAGC AGTCTCAGGC 000080
000081 CTTTAGTGAT GATGATTAAA GGTGGTGGCT GTGGCCTTGA AAACAGTCAT GTGAAAACTC ATCACCTTAA GGTGTTAAGT 000160
000161 GTAAGGATCT TCATGATGAA ATTTCTGTAA ATGGTGCAGT CAGCCTCAGT TTCCAAAGCC GGAAAAGGAT CCTCTAGTAG 000240
000241 CCACGGTGTG GCAGCTGCTC TGAACCAGGA CCTGGACCCG GACCCAAAGT GCCATGTCTT TAATGTGAGT TAGCTCCCAG 000320
000321 CGATGCCAGA TGGGATCAGC ACAGCCCTGC CTCTGCTGCT AATTGTTCCT CTAAAGTAAT CGCCATGCGT TCTTTGGGCT 000400
000401 TCATCTTTAA AGGAATGAAG CAACTGAGAT TATTCTGGAA AACCTTTTGG CAGTTAGTGA AATTAGAGTA CAACTAAGAA 000480
000481 CATTTTCAGA CCTCCACTGT GGATGACCTG GGTATAATCT CACAAATCGA TGGGACTGCA AGGATTGTAA ACTGAAATGA 000560
000561 ACATGATTAT ACTCTGTTGG AAGAGCCTAA GAGGAAACTG ATGCCATGAG TTTCAGAGAG TAATGCTTAA CCCCAGTTAC 000640
000641 ACAGGATGCC GTCTTGTGTT TCCTCTTGTT TAGTTACCCA CTACAGTGAT TTTGTGATCT GCTAATGGGT TGCCACCCAC 000720
000721 AACCATTGCT TTAGCACTTT TACTTCAAAT CAATGAAGGA TTGATAAAAG TTCTCCTGGT GTCTCCGCAG AGTGCCTTCC 000800
000801 AGGAACAGAT CTTTGCATAG AATATCAGTG GTTTCCTTTT TTGTTTCAAA TAGTGGTCAG AAAATACCCA GTGTTGACTC 000880
000881 ACCAAGGCAA TCAGCTTCCT TTTTCCCTTT TTTTGTTTTT TTTTTAACAT TTTATATTTT TGCTTTATTT TATTTTATTT 000960
000961 TATTTTATTT TATTTTATTT TATTTTATTT TTTGAGACGG AGTTCCACTC TGTCGCCAGG CTGGAGTGAA GTGGTACAAT 001040
001041 CTTGGCTCAC TGCAACCTCC ACCTCCCGGG TTCAAGCAAT TCTCCTGGCT CAGCCTCCTG AGTGCTGGGA CTACAGGCGC 001120
001121 GTACCTTCTT TAGTAGAGAC TGGGTTTCAC CATGTTGGCC AGGATGGTCT CTATCTCCTG ACCTTGTGAT CTGCCTGCCT 001200
001201 CAGCTTCCCA AAGTGCTGAG ATGACAGGTG TGAGCCATCA GACCCAGCAT TTTTTTTTTT AATTTAAATT TAAATTTTTT 001280
001281 TTCATTTTTT TGAGAGGTTT TTTTTGTTTT GTTTTGTTGT TGTTGTTGTT GTTGTTTTTG AGACAGTCTT GCTCTGTCAC 001360
001361 CCAGGCTGGG AGTGCAGTGG CATGATCTCT GCAACCTCTA CCTCCCAGGT TCAAGCAATT CTTGTGCCTC AGCCTCCCAA 001440
001441 GTAACTGGGA CTACAGGTGC ACGCTACCAC ACCTGGCTGA TTTTTTTTGT TTTAGTAGAG ACAGGGTTTC AACCATGTTG 001520
001521 CCCAGGTTGG TCTCAAACTC CTGAGCTCAG GCAATCCACC CGCCTTGGCC TCCCAAAGTG CTAGGATTAC AGGTGTGAGC 001600
001601 CACCACACCC AGCTATTTTT TCTTTCGTTT TTTAATTTTA AAGTTGGGGG GGGGTCTCAA TTTGTTACCC TGGCTGGTCT 001680
001681 CGAACTCCCG GACTTAAGCG ATCCTCTGGC TCCAAGCCCA CTACCAGTCT CAGGTTTCTT TACTAAAAGA TCACTACCTT 001760
001761 TTTTTCTCTT ATCTGCTGCC ATGTGAGATG TGGCTTTCAC CTTCCGCCAT GATTGTGAGG CCTTCCCAGC CATGTAGAAC 001840
001841 TGTAAGTCCA ATAAACCTCT TTTGTAAATT AAAAAAAAAA AATCACTATT TAAGATACTA GGATGGATTG TGACTGTTGA 001920
001921 GGAGTACTTA CATATCCTAC ATTTGACTAC ATTATTTCCA AACCAAGTAT TCCATCCAAA GGAACATACT GCTATCATAG 002000
002001 AGACCAAGGA GGGACTGTTT AAGGTTGCCA AGGTGAAGCG AGCTGAGAGG CTTTGTCCTC GTGCCAGTAA CTCTGAAATC 002080
002081 TCTCTTAATT CCTGCTGTCC AGGCAGCAGA ATGCCATGGT TTCCCCAAGT AGGTAGCTGC TTTAGCAGTT AAAGCCCAAA 002160
002161 TGTCTGTTCT GTTGATCAGA GGTCTCTGAA TTTCTGAAGT GGTGTTTCGT TTCTGGTGAC TGAGTTAATC CTTTACAATC 002240
002241 CCTCTTGTAA AGTGTGCTAA TAGAAAGAAT CCACCTTTCA AAGCTGCAGA ACCAGACCGT GCCCTAAATT GACCAACGTA 002320
002321 ACTGATGTGC CTCAGGAAGT CTCTTGCCAG CTGTCCCTGT GAAGACCCCC CTCCTCCCCC CCAGCTGCTG CCTTGCACAC 002400
002401 TGAAGCATCT CAGACTGTGC AAAGCCGTGT AGTCATCAAG ACAGTAAATC CCAGGGCTTG GTTAAGTGCT GTGTGATAAC 002480
002481 TTGTTTGGAT GAGACTTAAC TTAAAACCAC TTACAATAAA CTTGGGAAAC TACCGTCA