THORLNC
THOR is a conserved testis lncRNA involved in multiple human carcinomas.
Contents
Annotated Information
Name
THORLNC: testis associated oncogenic lncRNA (HGNC nomenclature).
Characteristics
THORLNC has a chromosomal locaton of 2q14.2 (HGNC data). Lnc-THOR is exclusively expressed in testis and human cancers [1].
Function
THORLNC has been reported to be involved in more than one cancer. In one study, THOR knockout produced fertilization defects in zebrafish and also conferred a resistance to melanoma onset [2]. THORLNC expression was also found to be essential for human osteosarcoma OS cell growth and therefore Lnc-THOR could be a novel therapeutic target and/or diagnosis marker for human OS [1]. THOR expression was also seen to promote Renal cell carcinoma (RCC) cell growth in vitro and in vivo and can be employed as a therapeutic target [3]. Moreover, knockdown of IGF2BP1-bound nc-THOR deplets IGF2BP1-dependent mRNAs, causing inhibition on A431 cell survival and proliferation and as a result IGF2BP1 silencing or knockout inhibited A431 tumor xenograft growth in mice [4].
Regulation
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Diseases
- Melanoma [2]
- Osteosarcoma (OS) [1]
- Renal cell carcinoma (RCC) [3]
- Skin squamous cell carcinoma (SSCC) [4]
Expression
Lnc-THOR expression is essential for human OS cell growth. Lnc-THOR could be a novel therapeutic target and/or diagnosis marker for human OS [1].THOR expression promotes RCC cell growth in vitro and in vivo. THOR could be a novel and important therapeutic target for human RCC [3].
| Experiment | Forward primer | Reverse primer |
|---|---|---|
| qRT-PCR | 5'-CAAGGTGCTTCTCTCTGGATTT- 3' | 5'-GCCAAAGTCATTTGTTGGGTAT- 3' [2] |
| qRT-PCR | 5'-CAAGGTGCTTCTCTCTGGATTT- 3' | 5'-GCCAAAGTCATTTGTTGGGTAT- 3' [4] |
Labs working on this lncRNA
- Michigan Center for Translational Pathology, University of Michigan, Ann Arbor, Michigan, USA. [2]
- Department of Cellular and Molecular Biology, University of Michigan, Ann Arbor, Michigan, USA. [2]
- Department of Pediatrics, Boston Children’s Hospital, Boston, Massachusetts, USA. [2]
- Department of Computational Medicine and Bioinformatics, Ann Arbor, Michigan, USA. [2]
- Department of Pathology, University of Michigan, Ann Arbor, Michigan, USA. [2]
- Ionis Pharmaceuticals, Inc. Carlsbad, California, USA. [2]
- Department of Dermatology, University of Michigan Medical School, Ann Arbor, Michigan, USA. [2]
- Comprehensive Cancer Center, University of Michigan, Ann Arbor, Michigan, USA. [2]
- Section of Thoracic Surgery, Department of Surgery, University of Michigan, Ann Arbor, Michigan, USA. [2]
- Department of Radiation Oncology, University of Michigan, Ann Arbor, Michigan, USA. [2]
- Department of Pediatrics and Communicable Diseases, University of Michigan. [2]
- Howard Hughes Medical Institute, University of Michigan, Ann Arbor, Michigan, USA. [2]
- Department of Urology, University of Michigan, Ann Arbor, Michigan, USA. [2]
- Department of Orthopaedics, Shaoxing People's Hospital, Shaoxing Hospital of Zhejiang University, Shaoxing, China. [1]
- Graduate School, Southern Medical University, Guangzhou, China; Department of Urology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China. [3]
- Department of Urology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China. [3]
- Department of Plastic Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, China [4]
- Department of Burn and Plastic Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China. [4]
References
- ↑ 1.0 1.1 1.2 1.3 1.4 Chen W, Chen M, Xu Y, Chen X, Zhou P, Zhao X, Pang F, Liang W. Long non-coding RNA THOR promotes human osteosarcoma cell growth in vitro and in vivo. Biochemical and biophysical research communications. 2018, 499(4):913-9.
- ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 Hosono Y, Niknafs YS, Prensner JR, Iyer MK, Dhanasekaran SM, Mehra R, Pitchiaya S, Tien J, Escara-Wilke J, Poliakov A, Chu SC. Oncogenic role of THOR, a conserved cancer/testis long non-coding RNA. Cell. 2017, 171(7):1559-72.
- ↑ 3.0 3.1 3.2 3.3 3.4 Ye XT, Huang H, Huang WP, Hu WL. LncRNA THOR promotes human renal cell carcinoma cell growth. Biochemical and biophysical research communications. 2018, 501(3):661-7.
- ↑ 4.0 4.1 4.2 4.3 4.4 Liu Z, Wu G, Lin C, Guo H, Xu J, Zhao T. IGF2BP1 over-expression in skin squamous cell carcinoma cells is essential for cell growth. Biochemical and biophysical research communications. 2018, 501(3):731-8.
Sequence
>gi|100506797|ref|NR_144530.1| Homo sapiens testis associated oncogenic lncRNA (HORLNC), long non-coding RNA
000081 CCGCACGAAA GCAAAAACAA AGCCATCTCT CCGGAGCAGA AATAGAACAG ACGTGGCCGG GGAAAGCCAA ATATTTCCTG 000160
000161 CCGTCCTGGT GAACACAATC GAGCAAGGCA GTGAAGCAAA CATCATTAGG TGGCTGGACT CAGACACATC ACGCTCCAGT 000240
000241 TTGGGTTCCA AGGTGCTTCT CTCTGGATTT TCACCTGCCT TGCCAAAAAT GATTGATTAC GCTGGGCCAA CTGGGTGTCC 000320
000321 GGCAATACCC AACAAATGAC TTTGGCCCCT AACAGCAGGT CTTGGCCAGA CAGAAATCAA TATTTCACCA TTAAAATCTA 000400
000401 TCAAAGAAGA GTTAAGGCAC CATCTGTCCC TGCCGCCTCT CTATGGTGTG TGAACATTAA ATCCGACACT TGTACTACAT 000480
000481 GGGTAATCAA TATATAGTGG TTCACAGATC ATTTGTGGTT CCCTTTTAGC AGTGATGGAT GTCTGGAATC GTTGTGATTC 000560
000561 ATTTTGTCAG ACCATTAAAC AAGATTCTGT CCAGTTCCTT CCTGATTTCA AAAAAAAAAA AAAAAAAAAA A
