Dynamics and signals of CD4+ T cell activation and the influencing factors revealed by single cell RNA-seq
| Title | Dynamics and signals of CD4+ T cell activation and the influencing factors revealed by single cell RNA-seq |
|---|---|
| Description | T cell activation is a key event in adaptive immunity. However, the effects of cell status, cell heterogeneity and cellular communication on T cell activation have not been systematically analyzed. We found resting CD4 T cells differentiated into conventional effector T cells (conv TEFF), cytokines high TEFF (cytohi TEFF), proliferation TEFF (prolif TEFF) and heat shock proteins (HSPs) high expressed T (HSPhi T) upon anti-CD3/CD28 stimulation. We called the stimulated T cells overlapped with resting T on UMAP as inert T. Different from resting T, inert T showed intermediate feature of T cell activation including expression of cytokines and activated T specific marker genes. Resting CD4 T cells were more likely to differentiate into cytohi TEFF and were less likely to differentiate into HSPhi T and Th0 in the presence of CD8 T cells. Resting CD4 T cells respond to anti-CD3/CD28 differently from either CD4 TN or CD4 TM, due to difference of cell states, cell-cell communications and specific microenvironment. |
| Organism | Homo sapiens |
| Data Type | Expression Profiling by NGS |
| Data Accessibility | Controlled-access |
| BioProject | PRJCA010895 |
| Release Date | 2022-08-08 |
| Submitter | Wenfei Jin (jinwf@sinh.ac.cn) |
| Organization | Shanghai Institute of Nutrition and Health, Chinese Academy of Sciences |
| Submission Date | 2022-08-03 |
The data cannot be downloaded as it has not yet been registered in the Human Genetic Resource Management Platform of MOST.
| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX001496-01 | cd3cd4_rawMatrix | 1 | Expression Profiling by NGS | 182.62 MB | tar | 0 | Unavailable |