Pre-mRNA processing factor 19 enhances esophageal squamous cell carcinoma progression by reprogramming fatty acid metabolism
| Title | Pre-mRNA processing factor 19 enhances esophageal squamous cell carcinoma progression by reprogramming fatty acid metabolism |
|---|---|
| Description | Liquid chromatography tandem mass spectrometry (LC-MS/MS)-based lipidomics were conducted in KYSE-150 cells with PRP19 siRNA or scramble siRNA (n = 6/group) using Ultra High-Performance Liquid Tandem Chromatography Quadrupole Time of Flight Mass Spectrometry (UHPLC-QTOFMS) (Biotree, Shanghai, China).The triple TOF mass spectrometer was used for its ability to acquire MS/MS spectra on an information-dependent basis (IDA) during an LC/MS experiment. In this mode, the acquisition software (Analyst TF 1.7, AB Sciex) continuously evaluates the full scan survey MS data as it collects and triggers the acquisition of MS/MS spectra depending on preselected criteria. In each cycle, the most intensive 12 precursor ions with intensity above 100 were chosen for MS/MS at collision energy of 45 eV (12 MS/MS events with accumulation time of 50 msec each). |
| Organism | Homo sapiens |
| Data Type | Lipidome Data by Mass Spectrometry (MS) |
| Data Accessibility | Controlled-access |
| BioProject | PRJCA013937 |
| Release Date | 2022-12-22 |
| Submitter | Guang-Cong Zhang (20111210054@fudan.edu.cn) |
| Organization | Zhongshan Hospital, Fudan University |
| Submission Date | 2022-12-20 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX002663-01 | nc_1 | 1 | Lipidome Data by Mass Spectrometry (MS) | 123.09 MB | mzXML | 0 | Controlled |
| OMIX002663-02 | si_3 | 1 | Lipidome Data by Mass Spectrometry (MS) | 128.68 MB | mzXML | 0 | Controlled |
| OMIX002663-03 | nc_4 | 1 | Lipidome Data by Mass Spectrometry (MS) | 123.6 MB | mzXML | 0 | Controlled |
| OMIX002663-04 | nc_2 | 1 | Lipidome Data by Mass Spectrometry (MS) | 124.52 MB | mzXML | 0 | Controlled |
| OMIX002663-05 | si_6 | 1 | Lipidome Data by Mass Spectrometry (MS) | 128.56 MB | mzXML | 0 | Controlled |
| OMIX002663-06 | nc_5 | 1 | Lipidome Data by Mass Spectrometry (MS) | 124.21 MB | mzXML | 0 | Controlled |
| OMIX002663-07 | si_1 | 1 | Lipidome Data by Mass Spectrometry (MS) | 125.82 MB | mzXML | 0 | Controlled |
| OMIX002663-08 | si_4 | 1 | Lipidome Data by Mass Spectrometry (MS) | 127.38 MB | mzXML | 0 | Controlled |
| OMIX002663-09 | si_5 | 1 | Lipidome Data by Mass Spectrometry (MS) | 129.85 MB | mzXML | 0 | Controlled |
| OMIX002663-10 | si_2 | 1 | Lipidome Data by Mass Spectrometry (MS) | 130.61 MB | mzXML | 0 | Controlled |
| OMIX002663-11 | nc_3 | 1 | Lipidome Data by Mass Spectrometry (MS) | 125.13 MB | mzXML | 0 | Controlled |
| OMIX002663-12 | nc_6 | 1 | Lipidome Data by Mass Spectrometry (MS) | 125.87 MB | mzXML | 0 | Controlled |