| Description |
Western blot was used to detect the effect of L001 on the proliferation of corneal epithelial cells treated with hyperosmolality. As a sensor of cellular nutrient, mTOR plays a central regulatory role in cell proliferation, growth, and differentiation. The results showed that compared with the HOsM group, the expression of p-mTOR, pS6K and p-4EBP in human cornea epithelial cell (HCECs) were significantly increased in L001 group and decreased in BPTES group, while the expression of p-AMPK and its downstream P-ACC were decreased in L001 group, which indicated that L001 can promote proliferation and inhibit apoptosis by improving energy metabolism of corneal epithelium. N: Nontreated control group; HOsM: Hypertonic treatment group; L001: L001 with Hypertonic treatment group; BPTES: BPTES+L001 with Hypertonic treatment group. |