Anti-CRISPR AcrIIC5 is a dsDNA mimic that inhibits type II-C Cas9 effectors by blocking PAM recognition
| Title | Anti-CRISPR AcrIIC5 is a dsDNA mimic that inhibits type II-C Cas9 effectors by blocking PAM recognition |
|---|---|
| Description | Anti-CRISPR proteins are encoded by phages to inhibit the CRISPR-Cas systems of the hosts. AcrIIC5 inhibits several naturally high-fidelity type II-C Cas9 enzymes, including orthologs from Neisseria meningitidis (Nme1Cas9) and Simonsiella muelleri (Smu-Cas9). Here, we solve the structure of AcrIIC5 in complex with Nme1Cas9 and sgRNA. We show that AcrIIC5 adopts a novel fold to mimic the size and charge distribution of double-stranded DNA, and uses its negatively charged grooves to bind and occlude the protospacer adjacent motif (PAM) binding site in the target DNA cleft of Cas9. |
| Organism | DNA-binding vector pODB80 |
| Data Type | Protein 3D Structure Data |
| Data Accessibility | Open-access |
| BioProject | PRJCA016730 |
| Release Date | 2023-05-05 |
| Submitter | Yunran Zhan (yunranzhan@ibp.ac.cn) |
| Organization | Institute of Biophysics Chinese Academy of Sciences |
| Submission Date | 2023-05-05 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX003906-01 | Crystal structure of the AcrIIC5 complex | 3 | Protein 3D Structure Data | 577.4 KB | zip | 0 |