| Title |
Anti-CRISPR AcrIIC5 is a dsDNA mimic that inhibits type II-C Cas9 effectors by blocking PAM recognition |
| Description |
Anti-CRISPR proteins are encoded by phages to inhibit the CRISPR-Cas systems of the hosts. AcrIIC5 inhibits several naturally high-fidelity type II-C Cas9 enzymes, including orthologs from Neisseria meningitidis (Nme1Cas9) and Simonsiella muelleri (Smu-Cas9). Here, we solve the structure of AcrIIC5 in complex with Nme1Cas9 and sgRNA. We show that AcrIIC5 adopts a novel fold to mimic the size and charge distribution of double-stranded DNA, and uses its negatively charged grooves to bind and occlude the protospacer adjacent motif (PAM) binding site in the target DNA cleft of Cas9. |
| Organism |
DNA-binding vector pODB80 |
| Data Type |
Protein 3D Structure Data |
| Data Accessibility |
Open-access |
| BioProject |
PRJCA016730 |
| Release Date |
2023-05-05 |
| Submitter |
Yunran Zhan (yunranzhan@ibp.ac.cn) |
| Organization |
Institute of Biophysics Chinese Academy of Sciences |
| Submission Date |
2023-05-05 |