Rational Design of a Miniature Photocatalytic CO2-Reducing Enzyme
| Title | Rational Design of a Miniature Photocatalytic CO2-Reducing Enzyme |
|---|---|
| Description | We designed a 33 kDa miniature photocatalytic CO2-reducing enzyme (mPCE) harboring a chromophore (BpC) and two [Fe4S4] clusters (FeA/FeB). Through reduction potential fine-tuning, we optimized the multiple-step electron hopping from BpC to FeA/FeB, culminating in a CO2/HCOOH conversion quantum efficiency of 1.43%. As mPCE can be overexpressed with a high yield in Escherichia coli cells without requiring synthetic cofactors, further development along this route may result in rapid photo-enzyme quantum yield improvement and functional expansion through an efficient directed evolution process. |
| Organism | unidentified |
| Data Type | Protein 3D Structure Data |
| Data Accessibility | Open-access |
| BioProject | PRJCA016795 |
| Release Date | 2023-05-09 |
| Submitter | Lin Liu (liulin@ibp.ac.cn) |
| Organization | Insititute of Biophysics, Chinese Academy of Sciences |
| Submission Date | 2023-05-08 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX003966-01 | mPCE relatated protein data | 2 | Protein 3D Structure Data | 85.2 KB | zip | 0 |