| Title |
Rational Design of a Miniature Photocatalytic CO2-Reducing Enzyme |
| Description |
We designed a 33 kDa miniature photocatalytic CO2-reducing enzyme (mPCE) harboring a chromophore (BpC) and two [Fe4S4] clusters (FeA/FeB). Through reduction potential fine-tuning, we optimized the multiple-step electron hopping from BpC to FeA/FeB, culminating in a CO2/HCOOH conversion quantum efficiency of 1.43%. As mPCE can be overexpressed with a high yield in Escherichia coli cells without requiring synthetic cofactors, further development along this route may result in rapid photo-enzyme quantum yield improvement and functional expansion through an efficient directed evolution process. |
| Organism |
unidentified |
| Data Type |
Protein 3D Structure Data |
| Data Accessibility |
Open-access |
| BioProject |
PRJCA016795 |
| Release Date |
2023-05-09 |
| Submitter |
Lin Liu (liulin@ibp.ac.cn) |
| Organization |
Insititute of Biophysics, Chinese Academy of Sciences |
| Submission Date |
2023-05-08 |