Neutralization ability of specific-IgG epitope peptide against SARS-CoV2 Pseudovirus
| Title | Neutralization ability of specific-IgG epitope peptide against SARS-CoV2 Pseudovirus |
|---|---|
| Description | We first predicted and synthesized several B cell epitopes on RBD and adjacent RBD of S protein, then the B cell epitopes were used to prepare affinity chromatography columns respectively and purify the binding IgG from serum samples of convalescent COVID19 patients. After these IgGs were identified to have neutralizing activity, the peptide sequences of the antigen-binding regions of neutralizing antibodies were analyzed by protein mass spectrometry. Subsequently, the B cells from the same individual were sorted and used to obtain their full BCR repertoire by 5' RACE combined with highthroughput of PacBio sequencing method. Then, the peptide sequence of neutralizing antibody variable region by protein mass spectrometry was mapped to the full BCR repertoire and found the full variable region sequence of neutralizing antibodies. Finally, we obtained and synthesized numerous CDR3 peptides of neutralizing antibodies to confirm the neutralizing activity for SARS-CoV-2 infection. |
| Organism | Homo sapiens |
| Data Type | Flow Cytometry |
| Data Accessibility | Controlled-access |
| BioProject | PRJCA018529 |
| Release Date | 2023-07-31 |
| Submitter | Jing Huang (huangjing82@bjmu.edu.cn) |
| Organization | School of Basic Medical Sciences |
| Submission Date | 2023-07-22 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX004578-01 | Neutralization ability of specific-IgG epitope peptide against SARS-CoV2 Pseudovirus | 1 | Flow Cytometry | 54.6 MB | zip | 0 | Controlled |