| Title |
Mass spectrometry based proteomic analysis of potential host proteins interacting with GP5 in PRRSV-infected PAMs |
| Description |
To understand the functions of porcine reproductive and respiratory syndrome virus (PRRSV) GP5, we identified GP5-interacting partners in PRRSV-infected primary alveolar macrophages (PAMs) by immunoprecipitation (IP) assay combined with LC-MS/MS. PAMs were infected with PRRSV, and then lysed for GP5-IP assay. After SDS-PAGE electrophoresis, the gel was revealed by silver staining, gel fragments were excised and identified by chromatography-tandem mass spectrometry (LC-MS/MS). |
| Organism |
Sus scrofa |
| Data Type |
Proteomic Data by Mass Spectrometry (MS) |
| Data Accessibility |
Open-access |
| BioProject |
PRJCA022547 |
| Release Date |
2024-03-26 |
| Submitter |
Wen Li (lw839466399@163.com) |
| Organization |
Henan Agricultural University |
| Submission Date |
2024-02-05 |