Transcriptomic sequencing of dMMR metastatic colorectal cancer
| Title | Transcriptomic sequencing of dMMR metastatic colorectal cancer |
|---|---|
| Description | Bulk RNA derived from 21 pre-treatment tumor tissue samples were extracted. Purified RNA was reverse transcribed to cDNA using NEBNext RNA First Strand Synthesis Module (NEB, Cat# E7525S) and NEBNext Ultra II nondirectional RNA Second Strand Synthesis Module (NEB, Cat# E6111S). Subsequently, the libraries were sequenced on a NovaSeq 6000 Sequencing System (Illumina) in PE150 mode. The obtained reads were first checked by fastqc and aligned to the hg19 reference human genome. For RNA-seq, filtered and aligned reads were processed using STAR (version 020201) and assembled using StringTie2 (version 1.3.5). |
| Organism | Homo sapiens |
| Data Type | Expression Profiling by NGS |
| Data Accessibility | Controlled-access |
| BioProject | PRJCA023640 |
| Release Date | 2024-05-31 |
| Submitter | Yanhong Deng (dengyanh@mail.sysu.edu.cn) |
| Organization | The Sixth Affiliated Hospital of Sun Yat-sen University |
| Submission Date | 2024-02-18 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX005845-01 | RNA Expression Matrix | 21 | Expression Profiling by NGS | 34.8 MB | xlsx | 0 | Controlled |