Transcriptomic sequencing of dMMR metastatic colorectal cancer
Title | Transcriptomic sequencing of dMMR metastatic colorectal cancer |
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Description | Bulk RNA derived from 21 pre-treatment tumor tissue samples were extracted. Purified RNA was reverse transcribed to cDNA using NEBNext RNA First Strand Synthesis Module (NEB, Cat# E7525S) and NEBNext Ultra II nondirectional RNA Second Strand Synthesis Module (NEB, Cat# E6111S). Subsequently, the libraries were sequenced on a NovaSeq 6000 Sequencing System (Illumina) in PE150 mode. The obtained reads were first checked by fastqc and aligned to the hg19 reference human genome. For RNA-seq, filtered and aligned reads were processed using STAR (version 020201) and assembled using StringTie2 (version 1.3.5). |
Organism | Homo sapiens |
Data Type | Expression Profiling by NGS |
Data Accessibility | Controlled-access |
BioProject | PRJCA023640 |
Release Date | 2024-05-31 |
Submitter | Yanhong Deng (dengyanh@mail.sysu.edu.cn) |
Organization | The Sixth Affiliated Hospital of Sun Yat-sen University |
Submission Date | 2024-02-18 |
The data cannot be downloaded as it has not yet been registered in the Human Genetic Resource Management Platform of MOST.
File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
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OMIX005845-01 | RNA Expression Matrix | 21 | Expression Profiling by NGS | 34.8 MB | xlsx | 0 | Unavailable |