Proteome sequencing by Mass Spectrometry (MS)
| Title | Proteome sequencing by Mass Spectrometry (MS) |
|---|---|
| Description | Total protein of samples was extracted with the Total Plant Protein Extraction Kit (MS) (BANGFEI, Beijing, China), and evaluated in content using the Bicinchoninic acid Protein Assay Reagent (Promega, WI, USA). Total proteins were digested by Trypsin Gold (Promega, WI, USA) with a ratio of 20:1 at 37°C for 12 h. The digested proteins were labeled with the isobaric tags for relative and absolute quantification (iTRAQ) tags using the eight-plex iTRAQ reagent (Applied Biosystems, CA, USA). The Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry (LC-MS/MS) experiment was performed on a Q Exactive Mass Spectrometer coupled to an Easy nLC (Thermo Fisher Scientific, Inc., MA, USA). |
| Organism | Elymus sibiricus |
| Data Type | Proteomic Data by Mass Spectrometry (MS) |
| Data Accessibility | Open-access |
| BioProject | PRJCA024069 |
| Release Date | 2024-03-08 |
| Submitter | lu Huan (luhuanhuan0925@126.com) |
| Organization | Lanzhou University |
| Submission Date | 2024-03-07 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX005948-01 | Proteome-seq_data | 6 | Proteomic Data by Mass Spectrometry (MS) | 1.07 GB | zip | 0 |