Human induced pluripotent stem cells (RNA-seq)
| Title | Human induced pluripotent stem cells (RNA-seq) |
|---|---|
| Description | Human induced pluripotent stem cells (iPSCs) are crucial for regenerating cells and modeling diseases, yet the process is inefficient due to a lack of understanding of human-specific reprogramming mechanisms. This study examines the changes in chromatin accessibility as human iPSCs are induced from urine cells, revealing a slower closure of somatic loci in humans compared to mice. A conserved AP-1 motif is significantly enriched in these closed regions. Introducing the AP-1 repressor JDP2 improves human reprogramming and the activation of pluripotent genes, whereas known pluripotency factors like ESRRB, KDM2B, and SALL4 do not enhance human iPSC generation. JDP2 promotes the closure of somatic loci with AP-1 motifs, facilitating human reprogramming, and can compensate for the absence of MYC or KLF4. The findings suggest that AP-1 activity is a key obstacle in chromatin remodeling during the reprogramming of somatic cells. |
| Organism | Homo sapiens |
| Data Type | Expression Profiling by NGS |
| Data Accessibility | Open-access |
| BioProject | PRJCA024307 |
| Release Date | 2024-07-01 |
| Submitter | Jiekai Chen (chen_jiekai@gibh.ac.cn) |
| Organization | Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences |
| Submission Date | 2024-03-15 |
HTTP download speed may be slow. It is highly recommended that you download the dataset using a dedicated FTP tool (such as FileZilla Client).
| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX006003-01 | Total mRNA was isolated from hUCs, the cells of reprogrammed on day 0, 1, 3, 6, 9, 12,15 and ESCs | 18 | Expression Profiling by NGS | 802.64 KB | gz | 0 |