Proteome dataset of Klac peptides from HeLa cells
| Title | Proteome dataset of Klac peptides from HeLa cells |
|---|---|
| Description | Briefly, equal amount of peptides was first dissolved in NETN buffer [100 mM NaCl, 50 mM tris-HCl, 1 mM EDTA, and 0.5% NP-40 (pH 8.0)] and incubated with pan anti-KLac beads (PTM BIO, PTM-1404) at 4°C overnight. Then, the beads were washed three times with NETN buffer and twice with ddH2O. The combined peptides were eluted with 0.1% (v/v) TFA. The isolated KLac peptides were dried in SpeedVac (Thermo Scientific) and were analyzed on a Q Exactive HF-X instrument (ThermoFisher) coupled with an Easy-nLC 1200 system. |
| Organism | Homo sapiens |
| Data Type | Proteomic Data by Mass Spectrometry (MS) |
| Data Accessibility | Open-access |
| BioProject | PRJCA024613 |
| Release Date | 2024-04-23 |
| Submitter | Zhang Long (l_zhang@zju.edu.cn) |
| Organization | Life Sciences Institute, Zhejiang University |
| Submission Date | 2024-04-04 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX006151-01 | HeLa_siAARS1_Lac | 1 | Proteomic Data by Mass Spectrometry (MS) | 997.29 MB | raw | 0 | |
| OMIX006151-02 | HeLa_siAARS2_Lac | 1 | Proteomic Data by Mass Spectrometry (MS) | 1.05 GB | raw | 0 | |
| OMIX006151-03 | HeLa_siAARS1 | 1 | Proteomic Data by Mass Spectrometry (MS) | 1.07 GB | raw | 0 | |
| OMIX006151-04 | HeLa_siAARS1/2_Lac | 1 | Proteomic Data by Mass Spectrometry (MS) | 824.94 MB | raw | 0 | |
| OMIX006151-05 | HeLa | 1 | Proteomic Data by Mass Spectrometry (MS) | 1.09 GB | raw | 0 | |
| OMIX006151-06 | HeLa_siAARS1/2 | 1 | Proteomic Data by Mass Spectrometry (MS) | 801.3 MB | raw | 0 | |
| OMIX006151-07 | HeLa_siAARS2 | 1 | Proteomic Data by Mass Spectrometry (MS) | 1010.06 MB | raw | 0 | |
| OMIX006151-08 | HeLa_Lac | 1 | Proteomic Data by Mass Spectrometry (MS) | 1.05 GB | raw | 0 |