Proteome dataset of lactylated proteins
| Title | Proteome dataset of lactylated proteins |
|---|---|
| Description | cGAS and mCherry was expressed in E. coli. After expression, the cells were harvested by centrifuging at 4000 rpm for 0.5 h. The cell pellets were resuspended in ice-cold buffer A (50 mM Tris, 200 mM NaCl, 10 mM imidazole, 1 mM β-mercaptoethanol, pH 8.0) and lysed by sonication. After centrifugation (12000 rpm, 1 h), the lysate was loaded into a Ni-NTA column and washed with 30 column volume (CV) of buffer A, then the lysate was washed with 5 CV of buffer B (50 mM Tris, 200 mM NaCl, 50 mM imidazole, 1 mM β-mercaptoethanol, pH 8.0). Finally, the proteins were eluted with 2 CV of buffer C (50 mM Tris, 200 mM NaCl, 500 mM imidazole, 1 mM β-mercaptoethanol, pH 8.0). Purified proteins were subjected to LC-MS analysis. For mass spectrometry assay, the purified proteins were analyzed on an Xevo G2-XS QTOF MS System (Waters Corporation) equipped with an electrospray ionization (ESI) source in conjunction with Waters ACQUITY UPLC I-Class plus. |
| Organism | Bacteria |
| Data Type | Proteomic Data by Mass Spectrometry (MS) |
| Data Accessibility | Open-access |
| BioProject | PRJCA024613 |
| Release Date | 2024-04-23 |
| Submitter | Zhang Long (l_zhang@zju.edu.cn) |
| Organization | Life Sciences Institute, Zhejiang University |
| Submission Date | 2024-04-04 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX006152-01 | lactylated-mcherry-protein-weight | 1 | Proteomic Data by Mass Spectrometry (MS) | 64.2 MB | raw | 0 | |
| OMIX006152-02 | lactylated-mcGAS-protein-weight | 1 | Proteomic Data by Mass Spectrometry (MS) | 240.24 MB | raw | 0 | |
| OMIX006152-03 | lactylated-hcGAS-protein-weight | 1 | Proteomic Data by Mass Spectrometry (MS) | 246.15 MB | raw | 0 | |
| OMIX006152-04 | mcherry-wt-protein-weight | 1 | Proteomic Data by Mass Spectrometry (MS) | 55.8 MB | raw | 0 | |
| OMIX006152-05 | lactylated-mcGAS-site | 1 | Proteomic Data by Mass Spectrometry (MS) | 1.01 GB | raw | 0 | |
| OMIX006152-06 | lactylated-hcGAS-site | 1 | Proteomic Data by Mass Spectrometry (MS) | 1.05 GB | raw | 0 | |
| OMIX006152-07 | mcherry-wt-site | 1 | Proteomic Data by Mass Spectrometry (MS) | 1.03 GB | raw | 0 | |
| OMIX006152-08 | lactylated-mcherry-site | 1 | Proteomic Data by Mass Spectrometry (MS) | 1.2 GB | raw | 0 |