| Title |
Proteome dataset of cGAS lactylation and interactome |
| Description |
For mass spectrometry of cGAS interaction proteins, HEK293T cells transfected with Flag-cGAS were harvested and followed with purification by immunoaffinity purification with anti-Flag beads or IgG control beads. The samples were separated with SDS-PAGE. SDS-PAGE gels were minimally stained with Coomassie brilliant blue, cut into 6 molecular weight ranges based on heavy chain IgG bands, and digested with trypsin. Immunocomplexes were identified on a Q Exactive HF-X instrument (ThermoFisher) coupled with an Easy-nLC 1200 system. For mass spectrometry of cGAS lactylation, after pretreatment with 25 mM sodium L-lactate for 24 hours, HEK293T cells transfected with Flag-cGAS were harvested and followed with purification by immunoaffinity purification with anti-Flag beads or IgG control beads. TImmunocomplexes were identified on a Q Exactive HF-X instrument (ThermoFisher) coupled with an Easy-nLC 1200 system. |
| Organism |
Homo sapiens |
| Data Type |
Proteomic Data by Mass Spectrometry (MS) |
| Data Accessibility |
Open-access |
| BioProject |
PRJCA024613 |
| Release Date |
2024-04-23 |
| Submitter |
Zhang Long (l_zhang@zju.edu.cn) |
| Organization |
Life Sciences Institute, Zhejiang University |
| Submission Date |
2024-04-04 |