Proteome dataset of cGAS lactylation and interactome
| Title | Proteome dataset of cGAS lactylation and interactome |
|---|---|
| Description | For mass spectrometry of cGAS interaction proteins, HEK293T cells transfected with Flag-cGAS were harvested and followed with purification by immunoaffinity purification with anti-Flag beads or IgG control beads. The samples were separated with SDS-PAGE. SDS-PAGE gels were minimally stained with Coomassie brilliant blue, cut into 6 molecular weight ranges based on heavy chain IgG bands, and digested with trypsin. Immunocomplexes were identified on a Q Exactive HF-X instrument (ThermoFisher) coupled with an Easy-nLC 1200 system. For mass spectrometry of cGAS lactylation, after pretreatment with 25 mM sodium L-lactate for 24 hours, HEK293T cells transfected with Flag-cGAS were harvested and followed with purification by immunoaffinity purification with anti-Flag beads or IgG control beads. TImmunocomplexes were identified on a Q Exactive HF-X instrument (ThermoFisher) coupled with an Easy-nLC 1200 system. |
| Organism | Homo sapiens |
| Data Type | Proteomic Data by Mass Spectrometry (MS) |
| Data Accessibility | Open-access |
| BioProject | PRJCA024613 |
| Release Date | 2024-04-23 |
| Submitter | Zhang Long (l_zhang@zju.edu.cn) |
| Organization | Life Sciences Institute, Zhejiang University |
| Submission Date | 2024-04-04 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX006153-01 | hcGAS_lactylation_site | 1 | Proteomic Data by Mass Spectrometry (MS) | 1.2 GB | raw | 0 | |
| OMIX006153-02 | mcGAS_lactylation_site | 1 | Proteomic Data by Mass Spectrometry (MS) | 414.13 MB | raw | 0 | |
| OMIX006153-03 | hcGAS_AARS_interactome | 1 | Proteomic Data by Mass Spectrometry (MS) | 434.13 MB | raw | 0 |