The effect of cold-tolerant lactic acid bacteria on the metabolic succession during low-temperature ensiling fermentation of Jerusalem artichokes from the Qinghai-Tibet Plateau
Title | The effect of cold-tolerant lactic acid bacteria on the metabolic succession during low-temperature ensiling fermentation of Jerusalem artichokes from the Qinghai-Tibet Plateau |
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Description | Samples stored at -80 were thawed at room temperature. Soil: Weigh 500 mg of sample and place it in a 2 mL centrifuge tube. Add two small steel beads followed by 1 mL of methanol-water (V:V=1:1, with L-2-chlorophenylalanine at 2 μg/mL). Pre-cool the sample at -40 for 2 min. Grind using a grinder at 60 Hz for 2 min. Transfer the homogenized sample to a 15 mL centrifuge tube and rinse the tube wall residue with 1 mL of methanol-water (V:V=1:1, containing L-2-chlorophenylalanine at 2 μg/mL). Repeat the procedure once. Centrifuge for 10 min at 7700 rpm and 4. Transfer 2.5 mL of the supernatant to a 5 mL centrifuge tube and freeze-dry. |
Organism | Helianthus tuberosus |
Data Type | Metabolome Data by Mass Spectrometry (MS) |
Data Accessibility | Open-access |
BioProject | PRJCA025135 |
Release Date | 2024-04-16 |
Submitter | Xiaoqiang Wei (13649729986@163.com) |
Organization | Qinghai University |
Submission Date | 2024-04-12 |
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File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
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OMIX006239-01 | DLM202212773 | 3 | Metabolome Data by Mass Spectrometry (MS) | 12.92 GB | tar | 0 |