Involvement of Mycobacterium smegmatis small noncoding RNA B11 in triacylglycerol accumulation and altered cell wall permeability
| Title | Involvement of Mycobacterium smegmatis small noncoding RNA B11 in triacylglycerol accumulation and altered cell wall permeability |
|---|---|
| Description | In this study, we demonstrated that overexpression of B11 significantly affects bacterial growth and colony morphology, increases antibiotic sensitivity and sodium dodecyl sulfate (SDS) surface stress, decreases intracellular survival, and suppresses cytokine secretion in macrophages. Transcriptomic and lipidomic analyses revealed a metabolic downshift in the B11 overexpression strain, characterized by reduced levels of TAG. Furthermore, transmission electron microscopy showed that the B11 overexpression strain exhibited decreased cell wall thickness, leading to reduced biofilm formation and altered cell wall permeability. Additionally, we observed that B11 regulated certain target genes but did not directly bind to proteins. |
| Organism | Mycolicibacterium smegmatis |
| Data Type | Lipidome Data by Mass Spectrometry (MS) |
| Data Accessibility | Open-access |
| BioProject | PRJCA028533 |
| Release Date | 2024-07-27 |
| Submitter | Zhuhua Wu (wzhgdtb@163.com) |
| Organization | center for Tuberculosis Control of Guangdong Province |
| Submission Date | 2024-07-26 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX006976-01 | S1_neg.mzXML | 1 | Lipidome Data by Mass Spectrometry (MS) | 40.4 MB | mzXML | 0 | |
| OMIX006976-02 | S2_neg.mzXML | 1 | Lipidome Data by Mass Spectrometry (MS) | 39.7 MB | mzXML | 0 | |
| OMIX006976-03 | S3_neg.mzXML | 1 | Lipidome Data by Mass Spectrometry (MS) | 40.08 MB | mzXML | 0 | |
| OMIX006976-04 | S4_neg.mzXML | 1 | Lipidome Data by Mass Spectrometry (MS) | 39.97 MB | mzXML | 0 | |
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| OMIX006976-07 | S7_neg.mzXML | 1 | Lipidome Data by Mass Spectrometry (MS) | 40.29 MB | mzXML | 0 | |
| OMIX006976-08 | S8_neg.mzXML | 1 | Lipidome Data by Mass Spectrometry (MS) | 39.56 MB | mzXML | 0 | |
| OMIX006976-09 | V1_neg.mzXML | 1 | Lipidome Data by Mass Spectrometry (MS) | 39.76 MB | mzXML | 0 | |
| OMIX006976-10 | V2_neg.mzXML | 1 | Lipidome Data by Mass Spectrometry (MS) | 39.84 MB | mzXML | 0 | |
| OMIX006976-11 | V3_neg.mzXML | 1 | Lipidome Data by Mass Spectrometry (MS) | 39.73 MB | mzXML | 0 | |
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