Construction of a differentiation system of human extraembryonic mesoderm cells and its production of mesenchymal stem cells with osteogenic tendency
| Title | Construction of a differentiation system of human extraembryonic mesoderm cells and its production of mesenchymal stem cells with osteogenic tendency |
|---|---|
| Description | Extraembryonic mesoderm cells (EXMCs) are involved in the development of multiple embryonic lineages and umbilical cord formation, where they subsequently develop into mesenchymal stem cells (MSCs). The present report described a three-dimensional differentiation protocol to induce hpESCs into EXMCs by activating the Wnt pathway using CHIR99021. Single-cell transcriptome and immunostaining analyses revealed that the EXMC characteristics were similar to those of post-implantation embryonic EXMCs. Cell sorting was used to purify and expand the EXMCs. Importantly, these EXMCs secreted extracellular matrix proteins, including COL3A1, and differentiated into MSCs. Inconsistent with other MSC types, these MSCs exhibited a strong differentiation potential for chondrogenic and osteogenic cells and lacked adipocyte differentiation. Together, these findings provided a protocol to generate EXMCs and subsequent MSCs from hpESCs. |
| Organism | Homo sapiens |
| Data Type | Proteomic Data by Mass Spectrometry (MS) |
| Data Accessibility | Controlled-access |
| BioProject | PRJCA031305 |
| Release Date | 2024-10-29 |
| Submitter | tianqing Li (litq@lpbr.cn) |
| Organization | Institute of Primate Translational Medicine, Kunming University of Science and Technology |
| Submission Date | 2024-10-25 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX007717-01 | UCMSC-P3-2 | 1 | Proteomic Data by Mass Spectrometry (MS) | 957.91 MB | raw | 0 | Controlled |
| OMIX007717-02 | EXMC-P3-3 | 1 | Proteomic Data by Mass Spectrometry (MS) | 914.61 MB | raw | 0 | Controlled |
| OMIX007717-03 | UCMSC-P3-3 | 1 | Proteomic Data by Mass Spectrometry (MS) | 946.15 MB | raw | 0 | Controlled |
| OMIX007717-04 | UCMSC-P3-1 | 1 | Proteomic Data by Mass Spectrometry (MS) | 941.81 MB | raw | 0 | Controlled |
| OMIX007717-05 | EXMC-P3-2 | 1 | Proteomic Data by Mass Spectrometry (MS) | 909.44 MB | raw | 0 | Controlled |
| OMIX007717-06 | EXMC-P3-1 | 1 | Proteomic Data by Mass Spectrometry (MS) | 888.71 MB | raw | 0 | Controlled |