Mass-spectrometry datasets generated for the immuno-precipitated samples with the HA-OSBP1 and parental lines of T. gondii.
| Title | Mass-spectrometry datasets generated for the immuno-precipitated samples with the HA-OSBP1 and parental lines of T. gondii. |
|---|---|
| Description | For proteomic analysis of IP samples, protein digestion was carried out using a modified FASP method. In brief, 100 µg of protein underwent reduction, alkylation , and washing to eliminate stains and SDS. The samples were then digested overnight with trypsin at 37°C, and the resulting peptides were collected and diluted with 0.1% formic acid for nanoLC-MS analysis. NanoLC separation was performed using a Waters (Milford, MA, USA) nanoAcquity nanoHPLC with a gradient elution method. Nanospray ESI-MS was conducted on a Thermo Q-Exactive high-resolution mass spectrometer (Thermo Scientific, Waltham, MA, USA). |
| Organism | Toxoplasma gondii |
| Data Type | Proteomic Data by Mass Spectrometry (MS) |
| Data Accessibility | Open-access |
| BioProject | PRJCA032042 |
| Release Date | 2024-11-07 |
| Submitter | Ti Ti (798430667@qq.com) |
| Organization | China Agricultural University |
| Submission Date | 2024-11-05 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX007797-01 | OSBP-Proteome | 4 | Proteomic Data by Mass Spectrometry (MS) | 1.51 GB | zip | 0 |