Gut microbiota induces dysspermatogenesis via microbial-derived phenylacetylglycine in Ggt1-deficient mice
| Title | Gut microbiota induces dysspermatogenesis via microbial-derived phenylacetylglycine in Ggt1-deficient mice |
|---|---|
| Description | This study demonstrates that male gamma-glutamyl transferase 1-deletion (Ggt1-/-) mice exhibits infertility, characterized by reduced germ and testicular Leydig cell numbers and increased abnormal sperm rates. Fecal metagenomics shows distinct gut microbiota composition alterations in Ggt1-/- mice and Ggt1-/- fecal microbiota transplantation (Ggt1-/--FMT) results in the similar phenotypes with Ggt1-/- mice including reduced germ cells and increased abnormal sperm rates. Moreover, gut microbial-derived metabolite phenylacetylglycine (PAGly) increases in Ggt1-/- and Ggt1-/--FMT mice by serum metabolomic analysis, and contributes to abnormal spermatogenesis via in vivo injection. Transcriptomic analysis unveils lowered expression of kallikrein family (Klk1bs) members in Ggt1-/- and Ggt1-/--FMT testes. |
| Organism | Mus musculus |
| Data Type | Metabolome Data by Mass Spectrometry (MS) |
| Data Accessibility | Open-access |
| BioProject | PRJCA032081 |
| Release Date | 2024-11-08 |
| Submitter | Fenge Li (lifener@mail.hzau.edu.cn) |
| Organization | Huazhong Agricultural University |
| Submission Date | 2024-11-07 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX007804-01 | WT_8822_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 46.59 MB | zip | 0 | |
| OMIX007804-02 | WT_8810_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 47.05 MB | zip | 0 | |
| OMIX007804-03 | KO_0220_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 48.65 MB | zip | 0 | |
| OMIX007804-04 | KO_0216_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 46.45 MB | zip | 0 | |
| OMIX007804-05 | WT_8820_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 46.71 MB | zip | 0 | |
| OMIX007804-06 | KO_0218_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 46.4 MB | zip | 0 | |
| OMIX007804-07 | WT_8807_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 47 MB | zip | 0 | |
| OMIX007804-08 | KO_0218_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 48.8 MB | zip | 0 | |
| OMIX007804-09 | KO_0206_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 46.38 MB | zip | 0 | |
| OMIX007804-10 | QC002_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 47.81 MB | zip | 0 | |
| OMIX007804-11 | WT_8835_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 46.4 MB | zip | 0 | |
| OMIX007804-12 | WT_8835_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 47.22 MB | zip | 0 | |
| OMIX007804-13 | QC001_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 47.55 MB | zip | 0 | |
| OMIX007804-14 | WT_8812_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 46.29 MB | zip | 0 | |
| OMIX007804-15 | QC002_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 46.7 MB | zip | 0 | |
| OMIX007804-16 | WT_8807_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 46.44 MB | zip | 0 | |
| OMIX007804-17 | KO_0206_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 48.53 MB | zip | 0 | |
| OMIX007804-18 | WT_8820_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 46.52 MB | zip | 0 | |
| OMIX007804-19 | KO_0220_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 46.1 MB | zip | 0 | |
| OMIX007804-20 | KO_0211_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 48.83 MB | zip | 0 | |
| OMIX007804-21 | KO_0211_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 46.07 MB | zip | 0 | |
| OMIX007804-22 | WT_8822_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 47.11 MB | zip | 0 | |
| OMIX007804-23 | QC001_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 45.86 MB | zip | 0 | |
| OMIX007804-24 | KO_0215_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 46.11 MB | zip | 0 | |
| OMIX007804-25 | WT_8810_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 46.49 MB | zip | 0 | |
| OMIX007804-26 | QC003_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 46.42 MB | zip | 0 | |
| OMIX007804-27 | WT_8812_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 46.51 MB | zip | 0 | |
| OMIX007804-28 | KO_0215_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 48.72 MB | zip | 0 | |
| OMIX007804-29 | KO_0216_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 48.63 MB | zip | 0 | |
| OMIX007804-30 | QC003_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 49.19 MB | zip | 0 | |
| OMIX007804-31 | QC02_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 40.57 MB | wiff.zip | 0 | |
| OMIX007804-32 | FMT_KO1_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 42.75 MB | wiff.zip | 0 | |
| OMIX007804-33 | FMT_WT4_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 42.85 MB | wiff.zip | 0 | |
| OMIX007804-34 | QC01_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 42.42 MB | wiff.zip | 0 | |
| OMIX007804-35 | FMT_WT3_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 42.32 MB | wiff.zip | 0 | |
| OMIX007804-36 | FMT_KO4_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 40.05 MB | wiff.zip | 0 | |
| OMIX007804-37 | FMT_KO4_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 43.26 MB | wiff.zip | 0 | |
| OMIX007804-38 | FMT_KO5_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 40.35 MB | wiff.zip | 0 | |
| OMIX007804-39 | FMT_WT3_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 41.06 MB | wiff.zip | 0 | |
| OMIX007804-40 | QC03_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 41.34 MB | wiff.zip | 0 | |
| OMIX007804-41 | FMT_KO5_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 43.72 MB | wiff.zip | 0 | |
| OMIX007804-42 | FMT_KO6_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 39.69 MB | wiff.zip | 0 | |
| OMIX007804-43 | FMT_WT2_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 42.53 MB | wiff.zip | 0 | |
| OMIX007804-44 | FMT_KO2_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 43.15 MB | wiff.zip | 0 | |
| OMIX007804-45 | FMT_WT4_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 41.51 MB | wiff.zip | 0 | |
| OMIX007804-46 | FMT_KO2_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 40.26 MB | wiff.zip | 0 | |
| OMIX007804-47 | FMT_WT6_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 43.06 MB | wiff.zip | 0 | |
| OMIX007804-48 | FMT_WT6_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 41.17 MB | wiff.zip | 0 | |
| OMIX007804-49 | QC02_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 42.42 MB | wiff.zip | 0 | |
| OMIX007804-50 | QC01_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 41.27 MB | wiff.zip | 0 | |
| OMIX007804-51 | FMT_KO6_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 43.24 MB | wiff.zip | 0 | |
| OMIX007804-52 | FMT_WT2_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 40.89 MB | wiff.zip | 0 | |
| OMIX007804-53 | FMT_KO3_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 39.91 MB | wiff.zip | 0 | |
| OMIX007804-54 | FMT_WT1_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 42.81 MB | wiff.zip | 0 | |
| OMIX007804-55 | FMT_WT5_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 41.58 MB | wiff.zip | 0 | |
| OMIX007804-56 | QC03_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 42.93 MB | wiff.zip | 0 | |
| OMIX007804-57 | FMT_KO3_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 43.24 MB | wiff.zip | 0 | |
| OMIX007804-58 | FMT_WT5_NEG | 1 | Metabolome Data by Mass Spectrometry (MS) | 42.87 MB | wiff.zip | 0 | |
| OMIX007804-59 | FMT_WT1_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 41.05 MB | wiff.zip | 0 | |
| OMIX007804-60 | FMT_KO1_POS | 1 | Metabolome Data by Mass Spectrometry (MS) | 40.66 MB | wiff.zip | 0 |