| Title |
Proteomic and acetylated modified proteomic analysis of keratinocytes treated with red light irradiation-1 |
| Description |
After the enrichment of acetylated peptides from total cellular proteins using 4D-DIA, LC-MS/MS high-resolution mass spectrometry detection was performed on a Bruker timsTOF HT mass spectrometer coupled with a nanoElute 2 liquid chromatography system. The DIA liquid chromatography elution gradient was as follows: a C18 analytical column (15 cm x 100 µm, 1.9 µm) was utilized with buffer A as 0.1% formic acid in water and buffer B as 0.1% formic acid in acetonitrile. Following peptide separation, DIA (data-independent acquisition) mass spectrometry analysis was conducted using the timsTOF HT mass spectrometer (Bruker). The raw DIA data was processed using Spectronaut Pulsar 18.7 (Biognosys) software, and the sequence file for the database search was uniprot-Mus musculus-10090-irt-2024.2.1.fasta. |
| Organism |
Mus musculus |
| Data Type |
Proteomic Data by Mass Spectrometry (MS) |
| Data Accessibility |
Open-access |
| BioProject |
PRJCA044911 |
| Release Date |
2026-10-07 |
| Submitter |
Fagnqing Deng (dfq_nwpu@163.com) |
| Organization |
Northwestern Polytechnical University |
| Submission Date |
2025-08-19 |