Single-cell multi-omics sequencing reveals the reprogramming defects in embryos generated by round spermatid injection
| Title | Single-cell multi-omics sequencing reveals the reprogramming defects in embryos generated by round spermatid injection |
|---|---|
| Description | Round spermatid injection (ROSI) technique holds great promise for clinical treatment of infertile men who were diagnosed as that round spermatids are the most mature germ cells. However, the mechanisms underlying the poor developmental potential of both human and mouse ROSI embryos and how to overcome it remains largely unknown. Here, we establish the gene expression, chromatin accessibility and DNA methylation landscapes of mouse ROSI embryos using a single-cell multi-omics approach. Comparative analysis reveals the molecular reprogramming barriers marked by these three layer profiling abnormalities in ROSI embryos despite successful reprogramming globally. We screen out a novel small compound-A366 that can increase the rate of ROSI embryos to blastocyst stage and healthy pub by about 2 times. Finally, we demonstrate that A366 exerts its roles by partial overcoming of the reprogramming barriers. |
| Organism | Mus musculus |
| Data Type | - |
| Data Accessibility | Open-access |
| BioProject | PRJCA005396 |
| Release Date | 2022-06-28 |
| Submitter | Xiaoyang Zhao (zhaoxiaoyang@smu.edu.cn) |
| Organization | Southern Medical University |
| Submission Date | 2021-06-10 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX355-60-01 | WCG and GCH txt.gz | 936 | DNA Methylation | 28.9 GB | tar | 0 | |
| OMIX355-20-02 | gene_expression_log2TPM.txt.gz | 1 | Transcriptomic | 16.1 MB | gz | 0 |