CREBRF p.Arg457Gln promotes obesity and improves insulin sensitivity in gene-modified pig models
| Title | CREBRF p.Arg457Gln promotes obesity and improves insulin sensitivity in gene-modified pig models |
|---|---|
| Description | To investigate the functional consequences of the CREBRF variant, we introduced the corresponding human mutation p.Arg457Gln into porcine genome by using a CRISPR/Cas9-mediated homologous recombination (HR)-dependent approach. The CREBRF p.Arg457Gln pig models displayed dramatically increased fat deposition, yet improved sensitivity to insulin. Transcriptome and metabolome analyses of subcutaneous white adipose tissues showed that the CREBRF p.Arg457Gln mutation promoted preadipocyte differentiation, which indicated that obesity was caused by increased number (hyperplasia) rather than size (hypertrophy) of adipocytes. This study provides further mechanistic insights into favourable adiposity resulting from CREBRF p.Arg457Gln. |
| Organism | Sus scrofa |
| Data Type | - |
| Data Accessibility | Open-access |
| BioProject | PRJCA006932 |
| Release Date | 2022-06-30 |
| Submitter | GIBH Biomedical Data & HPC (sciencedb@gibh.ac.cn) |
| Organization | Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences |
| Submission Date | 2021-10-22 |
HTTP download speed may be slow. It is highly recommended that you download the dataset using a dedicated FTP tool (such as FileZilla Client).
| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX703-20-01 | CREBRF related project-RNA-seq_result | 8 | Transcriptomic | 102.5 MB | zip | 0 | |
| OMIX703-30-02 | CREBRF related project-metabolite_result | 8 | Metabonomics | 28.03 MB | zip | 0 |