Systematic analyses of early-stage lung cancer by scRNA-seq and lipidomic reveals aberrant lipid metabolism as detection biomarkers
| Title | Systematic analyses of early-stage lung cancer by scRNA-seq and lipidomic reveals aberrant lipid metabolism as detection biomarkers |
|---|---|
| Description | Lung cancer is the leading cause of cancer mortality and early detection is the key to improve survival. However, there are no reliable blood-based tests currently available for early-stage lung cancer diagnosis. Here, we performed single-cell RNA sequencing of early-stage lung cancer and found lipid metabolism was broadly dysregulated in different cell types and glycerophospholipid metabolism is the most significantly altered lipid metabolism-related pathway. Untargeted lipidomics were detected in an exploratory cohort of 311 participants. Through support vector machine algorithm-based and mass spectrum-based feature selection, we have identified nine lipids as the most important detection features and developed a LC-MS-based targeted assay utilizing multiple reaction monitoring. |
| Organism | Homo sapiens |
| Data Type | - |
| Data Accessibility | Controlled-access |
| BioProject | PRJCA007120 |
| Release Date | 2021-11-09 |
| Submitter | Guangxi Wang (guangxiwang@bjmu.edu.cn) |
| Organization | Institute of Systems Biomedicine, Department of Pathology, School of Basic Medical Sciences, Peking-Tsinghua Center for Life Sciences, Peking University Health Science Center |
| Submission Date | 2021-11-09 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX728-40-01 | LC_NEG_3 | 71 | Lipidomics | 9.88 GB | zip | 0 | Controlled |
| OMIX728-40-02 | NC_NEG_2 | 70 | Lipidomics | 9.78 GB | zip | 0 | Controlled |
| OMIX728-40-03 | NC_POS_1 | 70 | Lipidomics | 5.54 GB | zip | 0 | Controlled |
| OMIX728-40-04 | LC_NEG_2 | 50 | Lipidomics | 7.07 GB | zip | 0 | Controlled |
| OMIX728-40-05 | LC_POS_2 | 50 | Lipidomics | 3.92 GB | zip | 0 | Controlled |
| OMIX728-40-06 | LC_POS_3 | 71 | Lipidomics | 5.72 GB | zip | 0 | Controlled |
| OMIX728-40-07 | NC_POS_2 | 70 | Lipidomics | 5.74 GB | zip | 0 | Controlled |
| OMIX728-40-08 | NC_NEG_1 | 70 | Lipidomics | 9.71 GB | zip | 0 | Controlled |
| OMIX728-40-09 | LC_POS_1 | 50 | Lipidomics | 3.81 GB | zip | 0 | Controlled |
| OMIX728-40-10 | LC_NEG_1 | 50 | Lipidomics | 7.79 GB | zip | 0 | Controlled |