The authors investigated the life cycle of Epstein-Barr virus (EBV) in keratinocytes of oral hairy leukoplakia by combining immunohistochemistry. DNA in situ hybridization, and lectin histochemistry with electron microscopy. Diffuse-staining components of the EBV early antigen complex (EA-D), EBV 150-kd capsid antigen (VCA), EBV membrane antigen (gp350/220), and double-stranded DNA were labeled with monoclonal antibodies. An EBV-DNA probe was used to locate EBV DNA. Wheat-germ agglutinin (WGA) was employed to distinguish Golgi-associated compartments. The authors found EBV proteins and EBV DNA only in keratinocytes with apparent viral assembly. In situ hybridization showed EBV DNA in free corelike material and in electron-dense cores of mature nucleocapsids. Monoclonal antibodies to nonspecific double-stranded DNA attached to the same structures and to marginated chromatin. Components of EA-D were dispersed throughout the nuclei but accumulated near condensed chromatin and in 'punched-out' regions of the chromatin. Epstein-Barr virus 150-kd capsid antigen was found only in the nuclei, where it appeared preferentially on mature nucleocapsids. As yet unexplained arrays of intranuclear particles that remained unlabeled with all EBV-specific probes reacted intensely with an antiserum against common papillomavirus antigen. Gp350/220 was detectable in various cellular membrane compartments and was highly concentrated on EBV envelopes in peripheral Golgi-associated secretory vesicles. It was less abundant on the extracellular EBV, indicating that viral membrane antigen partly dissociates from the mature virus. Combined lectin-binding histochemistry and electron microscopy demonstrated for the first time that EBV is processed in the Golgi apparatus, which eventually releases the virus by fusion with the plasma membrane. These results provide insight into the biologic events that occur during complete EBV replication in vivo.
Lancet. 1964 Mar 28;1(7335):702-3
[PMID:
14107961]
J Virol. 1969 Jul;4(1):75-93
[PMID:
5808113]
Intervirology. 1989;30(2):61-73
[PMID:
2542183]
Lancet. 1986 Feb 1;1(8475):240-2
[PMID:
2868257]
Oral Surg Oral Med Oral Pathol. 1986 Mar;61(3):249-55
[PMID:
3458127]
Oral Surg Oral Med Oral Pathol. 1988 Apr;65(4):421-6
[PMID:
3283634]
J Virol. 1985 Oct;56(1):328-32
[PMID:
2993664]
N Engl J Med. 1986 Apr 3;314(14):874-9
[PMID:
3005862]
Lancet. 1986 Nov 15;2(8516):1122-4
[PMID:
2877273]
Virchows Arch A Pathol Anat Histopathol. 1988;412(6):533-42
[PMID:
2834863]
J Virol. 1988 Aug;62(8):2614-21
[PMID:
2839689]
Gene. 1987;58(1):149-54
[PMID:
2826297]
Scand J Dent Res. 1988 Aug;96(4):353-9
[PMID:
2842854]
J Med Virol. 1988 Nov;26(3):271-7
[PMID:
2849634]
Dtsch Med Wochenschr. 1986 Sep 12;111(37):1394-7
[PMID:
3017674]
N Engl J Med. 1985 Dec 19;313(25):1564-71
[PMID:
2999595]
Arch Dermatol. 1987 May;123(5):624-8
[PMID:
3034172]
J Virol. 1987 Jun;61(6):1796-807
[PMID:
3033311]
Br Med Bull. 1985 Jan;41(1):75-9
[PMID:
2982449]
Virology. 1985 Feb;141(1):1-13
[PMID:
2983491]
J Oral Pathol. 1986 Oct;15(9):493-9
[PMID:
3100750]
Virchows Arch A Pathol Anat Histopathol. 1987;412(2):127-33
[PMID:
2447697]
Gene. 1986;42(3):303-12
[PMID:
2426158]
J Oral Pathol Med. 1989 Dec;18(10):565-72
[PMID:
2559979]
N Engl J Med. 1989 Jun 8;320(23):1559-60
[PMID:
2542792]
Science. 1984 Dec 14;226(4680):1322-5
[PMID:
6095452]
Lancet. 1984 Oct 13;2(8407):831-4
[PMID:
6148571]
J Cutan Pathol. 1986 Jun;13(3):235-41
[PMID:
3734224]
J Virol Methods. 1985 Mar;10(3):225-39
[PMID:
3886683]
J Gen Virol. 1984 Feb;65 ( Pt 2):397-404
[PMID:
6319581]
Science. 1983 Mar 11;219(4589):1225-8
[PMID:
6298935]
J Virol. 1984 Sep;51(3):795-8
[PMID:
6088802]
Int J Cancer. 1982 Jan 15;29(1):87-91
[PMID:
6277806]
Proc Natl Acad Sci U S A. 1982 Mar;79(5):1423-7
[PMID:
6280181]
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5307-11
[PMID:
6254073]
J Cell Biol. 1980 May;85(2):429-34
[PMID:
7372714]
J Histochem Cytochem. 1977 Nov;25(11):1187-200
[PMID:
21217]
J Gen Virol. 1978 Jul;40(1):103-19
[PMID:
211178]
Virology. 1974 Dec;62(2):444-57
[PMID:
4372787]
Biken J. 1971 Jun;14(2):177-90
[PMID:
4330685]
Virology. 1971 Feb;43(2):442-52
[PMID:
4322626]
J Natl Cancer Inst. 1976 Mar;56(3):485-8
[PMID:
176393]
Br J Cancer. 1973 Jun;27(6):434-40
[PMID:
4198465]
J Virol. 1976 May;18(2):727-37
[PMID:
178905]
J Virol. 1970 Feb;5(2):258-61
[PMID:
5464577]
Oral Surg Oral Med Oral Pathol. 1988 Mar;65(3):333-8
[PMID:
3162581]
