Expression profiling of abundant genes in pulmonary and cardiac muscle tissues of Tibetan Antelope (Pantholops hodgsonii).

Xiaomei Tong, Yingzhong Yang, Weiwei Wang, Zenzhong Bai, Lan Ma, Xiaoguang Zheng, Haibo Sun, Zhang Zhang, Mengyao Zhao, Jun Yu, Ri-Li Ge
Author Information
  1. Xiaomei Tong: Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101300, China.

Abstract

The Tibetan Antelope (TA), which has lived at high altitude for millions of years, was selected as the model species of high hypoxia-tolerant adaptation. Here we constructed two cDNA libraries from lung and cardiac muscle tissues, obtained EST sequences from the libraries, and acquired extensive expression data related energy metabolism genes. Comparative analyses of synonymous (Ks) and nonsynonymous (Ka) substitution rates of nucleus-encoded mitochondrial unigenes among different species revealed that many antelope genes have undergone rapid evolution. Surfactant-associated protein A (SP-A) and surfactant-associated protein B (SP-B) genes in the AT lineage experienced accelerated evolution compared to goat and sheep, and these two genes are highly expressed in the lung tissue. This study suggests that many specific genes of lung and cardiac muscle tissues showed unique expression profiles and may undergo fast adaptive evolution in TA. These data provide useful information for studying on molecular adaptation to high-altitude in humans as well as other mammals.

MeSH Term

Amino Acid Sequence
Animals
Antelopes
Databases, Nucleic Acid
Evolution, Molecular
Expressed Sequence Tags
Gene Expression Profiling
Gene Expression Regulation
Lung
Male
Molecular Sequence Data
Myocardium
Phylogeny
Pulmonary Surfactant-Associated Protein A
Pulmonary Surfactant-Associated Protein B
Sequence Alignment
Tibet

Chemicals

Pulmonary Surfactant-Associated Protein A
Pulmonary Surfactant-Associated Protein B

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