The two main proteins from the phloem exudate of Cucurbita maxima Duchesne have been isolated by ammonium-sulfate precipitation, DEAE-cellulose chromatography, and gel filtration, and have been characterized. They comprise about 40% each of the total protein. The amino-acid composition of these two proteins has been determined. Both are highly basic with an IEP above 9.5. The smaller protein has a molecular weight of ca. 30000 as determined by analytical ultracentrifugation, gel filtration, and SDS polyacrylamide gel electrophoresis. It easily dimerizes to a form which appears to be the naturally occurring structure. The larger protein has a molecular weight of 116000 (ultracentrifugation, SDS polyacrylamide gel electrophoresis). This protein forms a gel in the absence of SH-protecting agents. Several minor proteins have been detected by polyacrylamide gel electrophoresis.
Plant Physiol. 1969 Apr;44(4):497-502
[PMID:
16657091]
Biochim Biophys Acta. 1972 Feb 29;257(2):433-44
[PMID:
5022434]
J Cell Biol. 1974 Jan;60(1):221-35
[PMID:
4271979]
Hoppe Seylers Z Physiol Chem. 1962 Nov 15;329:278-88
[PMID:
13994792]
Planta. 1973 Sep;111(3):187-98
[PMID:
24469572]
Planta. 1967 Mar;74(1):18-44
[PMID:
24549870]
Planta. 1970 Mar;91(1):68-79
[PMID:
24499982]
Planta. 1971 Jun;99(2):154-62
[PMID:
24487552]
J Cell Biol. 1968 Aug;38(2):292-303
[PMID:
5664205]
Planta. 1973 Jun;109(2):147-52
[PMID:
24474060]
Anal Biochem. 1969 Jul;30(1):51-7
[PMID:
5791084]
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2028-31
[PMID:
5289362]
J Cell Biol. 1967 Sep;34(3):801-15
[PMID:
6050947]
Biochem Biophys Res Commun. 1967 Sep 7;28(5):815-20
[PMID:
4861258]
Planta. 1973 Sep;110(3):189-204
[PMID:
24474397]
Planta. 1971 Jun;99(2):179-82
[PMID:
24487555]
Planta. 1970 Mar;95(1):86-94
[PMID:
24497023]
Anal Biochem. 1969 Oct 15;32(1):185-90
[PMID:
5398264]
Planta. 1973 Mar;114(1):51-61
[PMID:
24458664]
Biochemistry. 1967 Jul;6(7):1948-54
[PMID:
6049437]
Planta. 1973 Mar;114(1):41-50
[PMID:
24458663]
Nat New Biol. 1971 Feb 3;229(5):152-3
[PMID:
4102009]
Planta. 1971 Sep;100(3):208-21
[PMID:
24488194]
J Biol Chem. 1973 Jul 10;248(13):4682-90
[PMID:
4268863]
Nat New Biol. 1971 Oct 27;233(43):283-4
[PMID:
5288127]
Anal Biochem. 1969 Jul;30(1):148-52
[PMID:
4183001]
Protoplasma. 1971;73(2):293-302
[PMID:
5126405]
Planta. 1972 Dec;106(4):369-73
[PMID:
24477311]
J Biol Chem. 1951 Nov;193(1):265-75
[PMID:
14907713]
Proc Natl Acad Sci U S A. 1971 Aug;68(8):1762-6
[PMID:
5288762]
J Cell Biol. 1968 Jul;38(1):25-39
[PMID:
5675415]
