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International journal of clinical and experimental medicine
2015;8 (5): 6926-36.

Schisandrin B inhibits the proliferation of human lung adenocarcinoma A549 cells by inducing cycle arrest and apoptosis.

Xue-Jiao Lv, Li-Jing Zhao, Yu-Qiu Hao, Zhen-Zhong Su, Jun-Yao Li, Yan-Wei Du, Jie Zhang
Author Information
  1. Xue-Jiao Lv: Department of Respiratory Medicine, The Second Affiliated Hospital of Jilin University Changchun 130041, Jilin, China.
  2. Li-Jing Zhao: Department of Pathophysiology, College of Basic Medicine, Jilin University Changchun 130021, Jilin, China.
  3. Yu-Qiu Hao: Department of Respiratory Medicine, The Second Affiliated Hospital of Jilin University Changchun 130041, Jilin, China.
  4. Zhen-Zhong Su: Department of Respiratory Medicine, The Second Affiliated Hospital of Jilin University Changchun 130041, Jilin, China.
  5. Jun-Yao Li: Department of Respiratory Medicine, The Second Affiliated Hospital of Jilin University Changchun 130041, Jilin, China.
  6. Yan-Wei Du: Department of Pathophysiology, College of Basic Medicine, Jilin University Changchun 130021, Jilin, China.
  7. Jie Zhang: Department of Respiratory Medicine, The Second Affiliated Hospital of Jilin University Changchun 130041, Jilin, China.
PMID: 26221229

Abstract

Lung cancer is the leading cause of cancer death in the world. Schizandrin B (Sch B) is one of the main dibenzocyclooctadiene lignans present in the fruit of Schisandra chinensis (Schisandraceae). Sch B has multiple functions against cancer. The aim of this study was to determine the effect of Sch B on the proliferation, cell cycling, apoptosis and invasion of lung adenocarcinoma A549 cells by MTT, flow cytometry, wound healing and transwell invasion assays. Treatment with Sch B inhibited the proliferation of A549 cells in a dose-dependent manner. Sch B induced cell cycle arrest at G0/G1 phase by down-regulating the expression of cyclin D1, cyclin-dependent kinase (CDK)4, and CDK6, but up-regulating p53 and p21 expression in A549 cells. Furthermore, Sch B triggered A549 cell apoptosis by increasing Bax, cleaved caspase-3, 9, Cyto C, but decreasing Bcl-2 and PCNA expression. In addition, Sch B inhibited the invasion and migration of A549 cells by down-regulating the expressions of HIF-1, VEGF, MMP-9 and MMP-2. Therefore, Sch B has potent anti-tumor activity and may be a promising traditional Chinese medicine for human lung carcinoma.

Keywords

A549 cells Schizandrin B cell cycling invasion migration proliferation

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