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Nature methods
Jan, 2016;13 (1): 87-93.

Fixed single-cell transcriptomic characterization of human radial glial diversity.

Elliot R Thomsen, John K Mich, Zizhen Yao, Rebecca D Hodge, Adele M Doyle, Sumin Jang, Soraya I Shehata, Angelique M Nelson, Nadiya V Shapovalova, Boaz P Levi, Sharad Ramanathan
Author Information
  1. Elliot R Thomsen: Allen Institute for Brain Science, Seattle, Washington, USA. ORCID
  2. John K Mich: Allen Institute for Brain Science, Seattle, Washington, USA.
  3. Zizhen Yao: Allen Institute for Brain Science, Seattle, Washington, USA.
  4. Rebecca D Hodge: Allen Institute for Brain Science, Seattle, Washington, USA.
  5. Adele M Doyle: Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA.
  6. Sumin Jang: Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA.
  7. Soraya I Shehata: Allen Institute for Brain Science, Seattle, Washington, USA.
  8. Angelique M Nelson: Allen Institute for Brain Science, Seattle, Washington, USA.
  9. Nadiya V Shapovalova: Allen Institute for Brain Science, Seattle, Washington, USA.
  10. Boaz P Levi: Allen Institute for Brain Science, Seattle, Washington, USA.
  11. Sharad Ramanathan: Allen Institute for Brain Science, Seattle, Washington, USA.
PMID: 26524239 DOI: 10.1038/nmeth.3629

Abstract

The diverse progenitors that give rise to the human neocortex have been difficult to characterize because progenitors, particularly radial glia (RG), are rare and are defined by a combination of intracellular markers, position and morphology. To circumvent these problems, we developed Fixed and Recovered Intact Single-cell RNA (FRISCR), a method for profiling the transcriptomes of individual fixed, stained and sorted cells. Using FRISCR, we profiled primary human RG that constitute only 1% of the midgestation cortex and classified them as ventricular zone-enriched RG (vRG) that express ANXA1 and CRYAB, and outer subventricular zone-localized RG (oRG) that express HOPX. Our study identified vRG and oRG markers and molecular profiles, an essential step for understanding human neocortical progenitor development. FRISCR allows targeted single-cell profiling of any tissues that lack live-cell markers.

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Grants

  1. T32 GM007598/NIGMS NIH HHS
  2. 5DP1MH099906-03/NCCDPHP CDC HHS
  3. R24 HD000836/NICHD NIH HHS
  4. DP1 MH099906/NIMH NIH HHS
  5. 5R24HD000836/NICHD NIH HHS

MeSH Term

Brain
Humans
Neuroglia
Single-Cell Analysis
Transcriptome
Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Links to CNCB-NGDC Resources

GEN: GEND000197 (Fixed single-cell transcriptomic characterization of human radial glial diversity)

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