Simultaneous quantification of picfeltarraenins IA and IB in rat plasma by UPLC-MS/MS: Application to a pharmacokinetic study.
Xin He, Yingjie Zhang, Hang Gao, Keyan Li, Yazhuo Zhang, Limin Sun, Guizhou Tao
Author Information
Xin He: Department of Cardiology, the First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, China.
Yingjie Zhang: Department of Cardiology, the First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, China.
Hang Gao: Department of Cardiology, the First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, China.
Keyan Li: Department of Cardiology, the First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, China.
Yazhuo Zhang: Department of Cardiology, the First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, China.
Limin Sun: Department of Cardiology, the First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, China.
Guizhou Tao: Department of Cardiology, the First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, China. Electronic address: taogzlmu@sina.com.
A simple and rapid quantitative UPLC-MS/MS method for simultaneous determination of picfeltarraenins IA and IB in rat plasma was developed and validated in accordance with the US FDA Bioanalytical Guidance (2001). Analytes were extracted from rat plasma by using methanol and separated on Agilent ZORBAX SB-C18 (50mm×2.1mm, 1.8μm) column by using a mobile phase composed of methanol and water (70:30, v/v). Eluents were monitored by ESI tandem mass spectrometry detection with SRM mode using ion transitions m/z 785.4→639.5, m/z 815.5→669.5, and m/z 763.5→455.3 for picfeltarraenin IA, picfeltarraenin IB, and internal standard, respectively. The method was validated over the linear range of 11.5-1150ng/mL and 13.0-1300ng/mL. The developed analytical method was applied to support a pharmacokinetic study on simultaneous estimation of picfeltarraenins IA and IB in rats.
