OpenLB
Open Library of Bioscience
Advanced Search
Biomedical chromatography : BMC
May, 2016;30 (5): 695-709.

Extraction, chromatographic and mass spectrometric methods for lipid analysis.

Sumitra Pati, Ben Nie, Robert D Arnold, Brian S Cummings
Author Information
  1. Sumitra Pati: Department of Pharmaceutical and Biomedical Sciences, University of Georgia, Athens, GA, 30602, USA.
  2. Ben Nie: Department of Drug Discovery and Development, Harrison School of Pharmacy, Auburn University, Auburn, AL, 36849, USA.
  3. Robert D Arnold: Department of Drug Discovery and Development, Harrison School of Pharmacy, Auburn University, Auburn, AL, 36849, USA.
  4. Brian S Cummings: Department of Pharmaceutical and Biomedical Sciences, University of Georgia, Athens, GA, 30602, USA.
PMID: 26762903 DOI: 10.1002/bmc.3683

Abstract

Lipids make up a diverse subset of biomolecules that are responsible for mediating a variety of structural and functional properties as well as modulating cellular functions such as trafficking, regulation of membrane proteins and subcellular compartmentalization. In particular, phospholipids are the main constituents of biological membranes and play major roles in cellular processes like transmembrane signaling and structural dynamics. The chemical and structural variety of lipids makes analysis using a single experimental approach quite challenging. Research in the field relies on the use of multiple techniques to detect and quantify components of cellular lipidomes as well as determine structural features and cellular organization. Understanding these features can allow researchers to elucidate the biochemical mechanisms by which lipid-lipid and/or lipid-protein interactions take place within the conditions of study. Herein, we provide an overview of essential methods for the examination of lipids, including extraction methods, chromatographic techniques and approaches for mass spectrometric analysis.

Keywords

chromatography extraction lipidomics mass spectrometry phospholipids

References

Can J Biochem Physiol. 1959 Aug;37(8):911-7 [PMID: 13671378]
Lipids. 1995 Feb;30(2):173-5 [PMID: 7769975]
Nucleic Acids Res. 2009 Jul;37(Web Server issue):W652-60 [PMID: 19429898]
J Chromatogr B Biomed Appl. 1995 Sep 15;671(1-2):169-95 [PMID: 8520691]
J Lipid Res. 1985 Jan;26(1):135-40 [PMID: 3973509]
J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Feb 25;785(1):89-99 [PMID: 12535842]
Biol Chem. 2006 Sep;387(9):1267-74 [PMID: 16972796]
Anal Chem. 2007 Jun 1;79(11):4083-93 [PMID: 17474710]
Anal Chem. 2011 Jul 15;83(14):5480-7 [PMID: 21634439]
Lipids. 1987 Apr;22(4):274-7 [PMID: 3600204]
J Lipid Res. 2008 May;49(5):1137-46 [PMID: 18281723]
Methods Enzymol. 2007;433:175-91 [PMID: 17954235]
Methods Mol Biol. 1993;19:153-67 [PMID: 7693219]
J Pharm Biomed Anal. 2004 Apr 1;35(1):199-206 [PMID: 15030895]
Chem Commun (Camb). 2006 Jul 21;(27):2872-4 [PMID: 17007401]
Lipids. 1987 Jul;22(7):480-9 [PMID: 3626775]
Prog Lipid Res. 2010 Oct;49(4):450-75 [PMID: 20643161]
Biochem Pharmacol. 2005 Nov 25;70(11):1697-706 [PMID: 16226224]
Angew Chem Int Ed Engl. 2009;48(44):8240-2 [PMID: 19746375]
J Mass Spectrom. 2000 Aug;35(8):953-66 [PMID: 10972995]
Angew Chem Int Ed Engl. 2010;49(5):873-6 [PMID: 20041465]
J Lipid Res. 2010 Mar;51(3):660-71 [PMID: 19767534]
J Chromatogr. 1987 Jun 12;395:33-46 [PMID: 3624355]
Nat Protoc. 2011 Jun 30;6(7):1060-83 [PMID: 21720319]
Proteomics Clin Appl. 2008 Oct;2(10-11):1435-43 [PMID: 21136792]
J Lipid Res. 2008 Jul;49(7):1477-87 [PMID: 18398221]
J Lipid Res. 1971 Mar;12(2):123-31 [PMID: 5554103]
Anal Biochem. 2001 Feb 15;289(2):202-16 [PMID: 11161314]
Nat Rev Mol Cell Biol. 2010 Aug;11(8):593-8 [PMID: 20606693]
Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2339-44 [PMID: 9122196]
Anal Biochem. 2003 Dec 1;323(1):84-93 [PMID: 14622962]
Anal Chem. 1995 Nov 1;67(21):3979-84 [PMID: 8633761]
Annu Rev Anal Chem (Palo Alto Calif). 2010;3:433-65 [PMID: 20636050]
Rapid Commun Mass Spectrom. 2005;19(5):654-66 [PMID: 15700236]
J Chromatogr B Biomed Sci Appl. 2000 Mar 31;740(1):101-7 [PMID: 10798299]
Prog Lipid Res. 2007 May-Jul;46(3-4):200-24 [PMID: 17540449]
Annu Rev Biophys. 2009;38:53-74 [PMID: 19086820]
Rapid Commun Mass Spectrom. 2009 Oct;23(19):3077-82 [PMID: 19705379]
J Oleo Sci. 2012;61(2):49-56 [PMID: 22277887]
Methods Enzymol. 2007;432:21-57 [PMID: 17954212]
Expert Rev Proteomics. 2005 Apr;2(2):253-64 [PMID: 15892569]
Angew Chem Int Ed Engl. 2006 Nov 6;45(43):7188-92 [PMID: 17001721]
J Biochem. 1987 Apr;101(4):997-1005 [PMID: 3611047]
J Proteome Res. 2007 Feb;6(2):552-8 [PMID: 17269712]
J Lipid Res. 1989 Sep;30(9):1471-3 [PMID: 2600547]
Prog Lipid Res. 2004 Sep;43(5):449-88 [PMID: 15458815]
Subcell Biochem. 2008;49:541-65 [PMID: 18751926]
Mol Biosyst. 2008 Feb;4(2):121-7 [PMID: 18213405]
Rapid Commun Mass Spectrom. 2007;21(3):421-8 [PMID: 17206744]
J Biol Chem. 1957 May;226(1):497-509 [PMID: 13428781]
Anal Chem. 2007 Mar 15;79(6):2525-36 [PMID: 17279727]
Anal Chem. 2006 Feb 15;78(4):1191-9 [PMID: 16478111]
Chem Biol. 2011 Mar 25;18(3):284-91 [PMID: 21439472]
J Am Soc Mass Spectrom. 1998 May;9(5):516-26 [PMID: 9879366]
Mol Nutr Food Res. 2013 Aug;57(8):1336-46 [PMID: 23729171]
J Chromatogr A. 2012 Jan 13;1220:26-34 [PMID: 22169191]
Chem Biol Interact. 2008 Aug 11;174(3):163-76 [PMID: 18602625]
Biochim Biophys Acta. 2011 Nov;1811(11):776-83 [PMID: 21757029]
Cell. 2010 Dec 10;143(6):888-95 [PMID: 21145456]
Front Biosci. 2007 Jan 01;12:2568-79 [PMID: 17127263]
Anal Chem. 2005 Apr 1;77(7):2166-75 [PMID: 15801751]
Anal Bioanal Chem. 2010 Feb;396(3):1273-80 [PMID: 19937430]
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Oct 1;877(27):2976-82 [PMID: 19646933]
PLoS Genet. 2007 Apr 27;3(4):e64 [PMID: 17465682]
J Chromatogr A. 2010 Jun 25;1217(26):4347-54 [PMID: 20452602]
Chem Rev. 1999 Oct 13;99(10):2977-3006 [PMID: 11749508]
Rapid Commun Mass Spectrom. 2009 Jul;23(14):2181-9 [PMID: 19530152]
Nat Biotechnol. 2003 Jul;21(7):813-7 [PMID: 12808461]
Anal Bioanal Chem. 2007 Oct;389(3):827-34 [PMID: 17673987]
J Lipid Res. 2009 Apr;50 Suppl:S317-22 [PMID: 19050313]
Anal Chem. 2006 Dec 15;78(24):8324-31 [PMID: 17165823]
Biomed Mass Spectrom. 1974 Apr;1(2):154-5 [PMID: 4433729]
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Oct 25;810(2):173-86 [PMID: 15380713]
J Pharm Biomed Anal. 2005 Feb 23;37(2):359-67 [PMID: 15708678]
J Chromatogr A. 2010 Aug 6;1217(32):5179-83 [PMID: 20598701]
Nature. 1996 Feb 1;379(6564):466-9 [PMID: 8559255]
Anal Chem. 1994 Nov 15;66(22):3977-82 [PMID: 7810900]
Anal Chem. 1997 Dec 1;69(23):4751-60 [PMID: 9406525]
Angew Chem Int Ed Engl. 2005 Nov 4;44(43):7094-7 [PMID: 16259018]
Mass Spectrom Rev. 2012 Jan-Feb;31(1):134-78 [PMID: 21755525]
Methods Enzymol. 1990;193:237-63 [PMID: 1706059]
J Chromatogr B Biomed Sci Appl. 2001 Jul 15;758(2):265-75 [PMID: 11486836]
Prog Lipid Res. 2008 Jan;47(1):15-36 [PMID: 17980916]
Eur J Mass Spectrom (Chichester). 2005;11(4):409-17 [PMID: 16204808]
Nat Methods. 2008 Jan;5(1):57-9 [PMID: 18084298]
Anal Chem. 1996 Jan 1;68(1):1-8 [PMID: 8779426]
Anal Chem. 2012 Jun 5;84(11):5035-9 [PMID: 22533540]
Biomed Chromatogr. 2006 Mar;20(3):227-43 [PMID: 16138296]
PLoS One. 2012;7(1):e29851 [PMID: 22272252]
Brief Funct Genomic Proteomic. 2008 Sep;7(5):395-409 [PMID: 18805902]
J Biol Chem. 2011 Jul 22;286(29):25427-33 [PMID: 21632539]
J Chromatogr A. 2013 Jul 12;1298:9-16 [PMID: 23743007]
J Chromatogr A. 2003 May 9;996(1-2):173-80 [PMID: 12830918]
Anal Bioanal Chem. 2009 Mar;393(6-7):1649-56 [PMID: 19194696]
Adv Perit Dial. 2000;16:36-41 [PMID: 11045258]
Talanta. 2009 Apr 15;78(1):1-18 [PMID: 19174196]
Cell Biochem Funct. 2004 Nov-Dec;22(6):389-98 [PMID: 15376232]
Science. 2004 Oct 15;306(5695):471-3 [PMID: 15486296]
Science. 2006 Mar 17;311(5767):1566-70 [PMID: 16543450]
Anal Biochem. 2007 Mar 15;362(2):155-67 [PMID: 17266916]
J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Jun 15;992:56-66 [PMID: 25958321]
Methods. 2006 Jun;39(2):104-11 [PMID: 16831558]
EMBO J. 2005 Sep 21;24(18):3159-65 [PMID: 16138081]
Mass Spectrom Rev. 2003 Sep-Oct;22(5):332-64 [PMID: 12949918]
Anal Chem. 2009 Aug 15;81(16):6668-75 [PMID: 19572562]
Methods Mol Biol. 2009;579:189-200 [PMID: 19763476]
J Proteome Res. 2010 May 7;9(5):2377-89 [PMID: 20355720]
Lipids. 2001 Apr;36(4):327-46 [PMID: 11383683]
Chem Soc Rev. 2009 Jul;38(7):1882-96 [PMID: 19551169]
J Agric Food Chem. 2006 Jun 14;54(12):4107-13 [PMID: 16756333]
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Aug 15;871(2):174-81 [PMID: 18458005]
J Am Soc Mass Spectrom. 2005 Feb;16(2):133-8 [PMID: 15694763]
Biochemistry. 2005 Dec 20;44(50):16684-94 [PMID: 16342958]
Mass Spectrom Rev. 2005 May-Jun;24(3):367-412 [PMID: 15389848]
J Pharm Biomed Anal. 2006 Oct 11;42(4):506-12 [PMID: 16762523]
Rapid Commun Mass Spectrom. 2004;18(18):2121-30 [PMID: 15317043]
PLoS One. 2007 Feb 14;2(2):e218 [PMID: 17299598]
Anal Biochem. 2002 Mar 15;302(2):191-8 [PMID: 11878797]
Anal Chem. 1979 Oct;51(12):1251-64 [PMID: 21902234]
J Mass Spectrom. 2007 Aug;42(8):1099-105 [PMID: 17621390]
Curr Opin Chem Biol. 2009 Dec;13(5-6):526-31 [PMID: 19744877]
Anal Chem. 2006 Jan 15;78(2):585-95 [PMID: 16408944]
Rapid Commun Mass Spectrom. 2003;17(19):2168-76 [PMID: 14515314]
Clin Chem. 2007 Sep;53(9):1684-93 [PMID: 17712005]
J Mass Spectrom. 2006 Jan;41(1):50-8 [PMID: 16287034]
Proc Natl Acad Sci U S A. 2009 May 5;106(18):7314-9 [PMID: 19366672]
Proteomics. 2014 Apr;14(7-8):924-35 [PMID: 24497498]
Bioinformatics. 2006 Mar 1;22(5):634-6 [PMID: 16403790]
Mass Spectrom Rev. 2007 Jul-Aug;26(4):606-43 [PMID: 17471576]
J Lipid Res. 2009 Apr;50 Suppl:S9-14 [PMID: 19098281]
Proc Natl Acad Sci U S A. 2009 Feb 17;106(7):2136-41 [PMID: 19174513]
J Lipid Res. 2010 Nov;51(11):3299-305 [PMID: 20671299]
Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10635-9 [PMID: 7938005]
J Lipid Res. 1994 Jun;35(6):1102-14 [PMID: 8077849]
J Lipid Res. 2006 Apr;47(4):864-79 [PMID: 16449763]
J Proteome Res. 2006 Jun;5(6):1484-7 [PMID: 16740000]

Grants

  1. R01 EB016100/NIBIB NIH HHS
  2. 1R01EB016100/NIBIB NIH HHS

MeSH Term

Chemical Fractionation
Chromatography
Lipids
Mass Spectrometry

Chemicals

Lipids
Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Review

Word Cloud

Similar Articles

Cited By