Jujuboside A is a kind of the saponins isolated from the seeds of Ziziphus jujuba, which possesses multiple biological effects, such as antianxiety, antioxidant, and anti-inflammatory effects; however, its mediatory effect on isoproterenol-stimulated cardiomyocytes has not been investigated yet. In this study, we tried to detect the protective effect and potential mechanism of JUA on ISO-induced cardiomyocytes injury. H9C2 cells were treated with ISO to induce cell damage. Cells were pretreated with JUA to investigate the effects on the cell viability, morphological changes, light chain 3 conversion, and the activation of PI3K/Akt/mTOR signaling pathway. Results showed that ISO significantly inhibited the cell viability in a time- and dose-dependent manner. JUA pretreatment could reverse the reduction of cell viability and better the injury of H9C2 cells induced by ISO. Western blot analysis showed that JUA could accelerate the phosphorylation of PI3K, Akt, and mTOR. Results also indicated that JUA could significantly decrease the ratio of microtubule-associated protein LC3-II/I in H9C2 cells. Taken together, our research showed that JUA could notably reduce the damage cause by ISO via promoting the phosphorylation of PI3K, Akt, and mTOR and inhibiting LC3 conversion, which may be a potential choice for the treatment of heart diseases.
Physiol Res. 2012;61(3):287-97
[PMID:
22480422]
Annu Rev Genet. 2009;43:67-93
[PMID:
19653858]
Biochim Biophys Acta. 2008 Jan;1784(1):159-85
[PMID:
17997386]
Eur J Pharmacol. 2014 Sep 5;738:206-13
[PMID:
24886882]
PLoS One. 2014 Feb 26;9(2):e88203
[PMID:
24586308]
Oncogene. 2004 Apr 19;23(18):3151-71
[PMID:
15094765]
Genome Biol. 2011 Jul 27;12(7):226
[PMID:
21867568]
Cell. 2010 Feb 5;140(3):313-26
[PMID:
20144757]
Semin Cell Dev Biol. 2004 Apr;15(2):147-59
[PMID:
15209374]
Eur J Pharmacol. 2013 Jan 15;699(1-3):213-8
[PMID:
23178800]
PLoS One. 2013;8(1):e55298
[PMID:
23383143]
Pharmacol Res. 2004 Sep;50(3):231-6
[PMID:
15225664]
J Biomol Struct Dyn. 2008 Aug;26(1):57-64
[PMID:
18533726]
Cancer Res. 2011 Jul 1;71(13):4573-84
[PMID:
21602434]
Mol Cell Biochem. 2011 Feb;348(1-2):129-39
[PMID:
21069433]
J Agric Food Chem. 2013 Apr 10;61(14):3351-63
[PMID:
23480594]
Eur J Pharmacol. 2012 Feb 29;677(1-3):116-22
[PMID:
22178923]
Ann Oncol. 2005 Apr;16(4):525-37
[PMID:
15728109]
Eur J Pharmacol. 2010 Oct 10;644(1-3):160-8
[PMID:
20624385]
Cell Death Dis. 2011 Oct 13;2:e217
[PMID:
21993393]
Chem Biol Interact. 2008 Apr 15;172(3):245-52
[PMID:
18294627]
Cell Death Differ. 2002 Feb;9(2):99-100
[PMID:
11840159]
Phytother Res. 2009 Oct;23(10):1361-6
[PMID:
19306480]
Nat Rev Mol Cell Biol. 2012 Feb 23;13(3):195-203
[PMID:
22358332]
Autophagy. 2009 Jul;5(5):585-9
[PMID:
19411822]
Life Sci. 2004 Jun 18;75(5):537-48
[PMID:
15158364]
Int J Cardiol. 2013 Oct 3;168(3):1943-50
[PMID:
23357048]
J Ethnopharmacol. 2010 Mar 24;128(2):419-23
[PMID:
20083184]
Am J Health Syst Pharm. 2010 Dec 15;67(24):2095-106
[PMID:
21116000]
J Ethnopharmacol. 2010 Jul 6;130(1):163-6
[PMID:
20347951]
Acta Pharmacol Sin. 2001 Nov;22(11):986-90
[PMID:
11749788]
J Ethnopharmacol. 2015 Jan 15;159:215-23
[PMID:
25449456]
J Pharm Biomed Anal. 2008 Dec 15;48(5):1467-70
[PMID:
18977107]
Cancer Res. 2005 Apr 15;65(8):3336-46
[PMID:
15833867]
J Ethnopharmacol. 2013 Mar 7;146(1):347-54
[PMID:
23333311]
Oncology. 2013;84(1):43-56
[PMID:
23128843]
Oncotarget. 2011 Mar;2(3):135-64
[PMID:
21411864]
Mol Biol Cell. 2004 Mar;15(3):1101-11
[PMID:
14699058]
