LC-MS/MS Analysis of Bile Acids.

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Sabrina Krautbauer, Gerhard Liebisch

Author Information

Sabrina Krautbauer: Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany.
Gerhard Liebisch: Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany. gerhard.liebisch@ukr.de.

PMID: 29363069 DOI: 10.1007/978-1-4939-7592-1_8

Besides their role as lipid solubilizers, bile acids (BAs) are increasingly appreciated as bioactive molecules. They bind to G-protein-coupled receptors and nuclear hormone receptors. So they control their own metabolism and act on lipid and energy metabolism. Here we describe a simple, accurate, and fast liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of BAs in human plasma/serum.

Bile acids Electrospray ionization Internal standards Isomer separation Liquid chromatography Method Quantification Tandem mass spectrometry

Bile Acids and Salts

Chromatography, Liquid

Humans

Isotope Labeling

Reproducibility of Results

Tandem Mass Spectrometry

Bile Acids and Salts

Journal Article Research Support, Non-U.S. Gov't

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