G78 nitrogen deposition collector was used to investigate the background value of local nitrogen deposition flux in Daiyun Mountain National Nature Reserve of Fujian province. The results showed that dry and wet nitrogen deposition of Daiyun Mountain National Nature Reserve was 2.30 kg·hm and 14.79 kg·hm from March to October in 2015. 53% of dry deposition was in the form of dissolved organic nitrogen (DON, 1.21 kg·hm). The main form of inorganic nitrogen (DIN) in dry deposition was NO deposition (0.71 kg·hm), with NH deposition (0.37 kg·hm) being lower compared to NO deposition. DON and DIN flux in wet deposition was 5.38 kg·hm and 9.41 kg·hm, respectively. DIN flux in wet deposition was mainly in the form of NH deposition (6.07 kg·hm). Wet nitrogen deposition flux was found positively correlated with local precipitation. Wet nitrogen deposition in Daiyun Mountain National Nature Reserve might have significant negative effects on local water resource.