BACKGROUND: Apogamy is a unique asexual reproduction in the ferns, in which somatic cells of gametophytes go through dedifferentiation and then differentiate into haploid sporophytes bypassing fertilization. Restricted to the lack of genomic information, molecular mechanisms of apogamy have remained unclear. Comparative transcriptome analysis was conducted at six stages between sexual reproduction and apogamy in the fern Adiantum reniforme var. sinense, in an effort to identify genes and pathways that might initiate the asexual reproduction.
RESULTS: Approximately 928 million high-quality clean reads were assembled into 264,791 unigenes with an average length of 615 bp. A total of 147,865 (55.84%) unigenes were successfully annotated. Differential genes expression analysis indicated that transcriptional regulation was more active in the early stage of apogamy compared to sexual reproduction. Further comparative analysis of the enriched pathways between the early stages of the two reproductive modes demonstrated that starch and sucrose metabolism pathway responsible for cell wall was only significantly enriched in asexual embryonic cell initiation. Furthermore, regulation of plant hormone related genes was more vigorous in apogamy initiation.
CONCLUSION: These findings would be useful for revealing the initiation of apogamy and further understanding of the mechanisms related to asexual reproduction.
BMC Plant Biol. 2010 Oct 25;10:230
[PMID:
20973995]
Plant Cell Rep. 2018 Feb;37(2):293-306
[PMID:
29080908]
Biochem Soc Trans. 2005 Feb;33(Pt 1):280-2
[PMID:
15667326]
BMC Genomics. 2011 Feb 08;12:99
[PMID:
21303537]
Plant Cell Rep. 2006 Dec;25(12):1300-7
[PMID:
16874526]
Plant Cell Rep. 2006 Mar;25(2):85-91
[PMID:
16408178]
Plant Physiol. 2011 Sep;157(1):40-54
[PMID:
21757635]
Bioinformatics. 2005 Oct 1;21(19):3787-93
[PMID:
15817693]
Nucleic Acids Res. 2008 Jan;36(Database issue):D480-4
[PMID:
18077471]
Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9440-5
[PMID:
12883005]
Biochemistry (Mosc). 2004 Mar;69(3):233-47
[PMID:
15061689]
Plant Signal Behav. 2013 Sep;8(9):
[PMID:
23857350]
Plant Physiol. 2004 Jun;135(2):969-77
[PMID:
15181208]
Sex Plant Reprod. 2012 Dec;25(4):293-304
[PMID:
23007856]
Plant Cell. 1991 Apr;3(4):419-30
[PMID:
1840920]
Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:431-444
[PMID:
15012296]
Nat Biotechnol. 2010 May;28(5):511-5
[PMID:
20436464]
Front Plant Sci. 2017 Mar 22;8:336
[PMID:
28382042]
Plant J. 2006 Apr;46(1):69-84
[PMID:
16553896]
Nat Biotechnol. 2011 May 15;29(7):644-52
[PMID:
21572440]
Physiol Plant. 2001 Mar;111(3):381-388
[PMID:
11240923]
Plant Physiol. 2016 Dec;172(4):2403-2415
[PMID:
27794102]
Plant Physiol. 2008 Jan;146(1):97-107
[PMID:
17981987]
Plant Biotechnol J. 2004 Jan;2(1):71-82
[PMID:
17166144]
Nucleic Acids Res. 2008 Jun;36(10):3420-35
[PMID:
18445632]
Plant Signal Behav. 2009 Sep;4(9):887-9
[PMID:
19847119]
Nature. 2006 May 11;441(7090):227-30
[PMID:
16688177]
Plant J. 2014 Sep;79(6):1020-1032
[PMID:
24961590]
Bioinformatics. 2003 Jan 22;19(2):307-8
[PMID:
12538263]
Plant Signal Behav. 2011 Apr;6(4):494-500
[PMID:
21406974]
Plant J. 2017 Apr;90(1):122-132
[PMID:
28078730]
Proc Natl Acad Sci U S A. 2009 Aug 4;106(31):13118-23
[PMID:
19625620]
Plant Cell Physiol. 2015 Jan;56(1):e5
[PMID:
25480117]
Plant Cell Rep. 1995 Jun;14(9):598-602
[PMID:
24185605]
Planta. 1973 Mar;111(1):85-90
[PMID:
24469421]
PLoS One. 2015 Oct 02;10(10):e0139466
[PMID:
26431530]
Plant Cell. 2012 Jun;24(6):2635-48
[PMID:
22669881]
Annu Rev Plant Biol. 2002;53:377-98
[PMID:
12221981]
Nat Commun. 2017 Jul 13;8(1):72
[PMID:
28706187]
Proc Natl Acad Sci U S A. 2009 Sep 22;106(38):16321-6
[PMID:
19805300]
Genome Biol. 2010;11(10):R106
[PMID:
20979621]
Mol Biol Rep. 2010 Jun;37(5):2493-507
[PMID:
19705297]
Plant Cell Rep. 2017 Jun;36(6):843-858
[PMID:
28255787]
Plant Cell. 2006 Dec;18(12):3399-414
[PMID:
17194763]
PLoS One. 2015 Mar 25;10(3):e0120669
[PMID:
25807295]
J Chem Biol. 2009 Nov;2(4):177-90
[PMID:
19763658]
Adiantum
Biosynthetic Pathways
Computational Biology
Gene Expression Profiling
Gene Expression Regulation, Plant
Gene Ontology
Genomics
Germ Cells, Plant
Molecular Sequence Annotation
Phenotype
Reproducibility of Results
Reproduction
Transcriptome
