Investigation about the Occurrence of Transmission Cycles of Arbovirus in the Tropical Forest, Amazon Region.
Pedro A Araújo, Maria O Freitas, Jannifer Oliveira Chiang, Franko Arruda Silva, Liliane Leal Chagas, Samir Mansour Casseb, Sandro Patroca Silva, Joaquim Pinto Nunes-Neto, José Wilson Rosa-Júnior, Bruna Sena Nascimento, Leonardo Almeida Hernández, Thito Bezerra Paz, Landeson L Barros, Eliana P Silva, Raimunda S Azevedo, Lívia C Martins, Milene S Ferreira, Pedro F Vasconcelos
Author Information
Pedro A Araújo: Institute of Biological Sciences, Federal University of Pará, Belém, PA 66075-110, Brazil. pedroaraujo@iec.gov.br. ORCID
Maria O Freitas: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil.
Jannifer Oliveira Chiang: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil.
Franko Arruda Silva: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil.
Liliane Leal Chagas: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil.
Samir Mansour Casseb: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil.
Sandro Patroca Silva: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil.
Joaquim Pinto Nunes-Neto: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil.
José Wilson Rosa-Júnior: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil.
Bruna Sena Nascimento: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil.
Leonardo Almeida Hernández: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil.
Thito Bezerra Paz: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil.
Landeson L Barros: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil.
Eliana P Silva: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil.
Raimunda S Azevedo: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil.
Lívia C Martins: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil.
Milene S Ferreira: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil.
Pedro F Vasconcelos: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil.
Because of its ecological characteristics, the Caxiuanã National Forest (FLONA) is a potential area of arbovirus circulation. The present study aimed to investigate the occurrence of arbovirus transmission cycles at FLONA de Caxiuanã. Five field trips were performed to capture mosquitoes and sylvatic vertebrates. For these vertebrates, we attempted viral isolation by cell monolayer inoculation from blood, and hemagglutination inhibition and further seroneutralization assays from sera. For mosquitoes, we performed tests of viral genome detection. A total of 338 vertebrates were captured, and the greatest representative was birds (251/74.26%). A total of 16,725 mosquitoes were captured, distributed among 56 species. There were no viruses isolated by newborn mouse inoculation. Among birds, antibodies against were the most prevalent. , , and were the most prevalent among mammals and reptiles. Fragments of , , , and genome were detected in a pool of mosquito samples. These results of the study suggest the occurrence of arbovirus transmission cycles in the FLONA of Caxiuanã. The proximity of human populations with elements, involved in transmission cycles, makes surveillance necessary in this population to avoid dispersion of arboviruses to naïve locations.
