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Methods in molecular biology (Clifton, N.J.)
2019;2035 157-176.

NMR Studies of G-Quadruplex Structures and G-Quadruplex-Interactive Compounds.

Clement Lin, Jonathan Dickerhoff, Danzhou Yang
Author Information
  1. Clement Lin: Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University, West Lafayette, IN, USA.
  2. Jonathan Dickerhoff: Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University, West Lafayette, IN, USA.
  3. Danzhou Yang: Department of Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University, West Lafayette, IN, USA. yangdz@purdue.edu.
PMID: 31444749 DOI: 10.1007/978-1-4939-9666-7_9

Abstract

G-quadruplexes are noncanonical, four-stranded nucleic acid secondary structures formed in sequences containing consecutive runs of guanines. These G-quadruplex structures have been found to form in nucleic acid regions of biological significance, including human telomeres, gene promoters, and untranslated regions of mRNA. Thus, they are considered attractive therapeutic targets. Nuclear magnetic resonance (NMR) spectroscopy is a powerful method for understanding the structures of G-quadruplexes and their interactions with small molecules under physiologically relevant conditions. Here, we present the NMR methodology used in our research group for the study of DNA G-quadruplex structures in physiologically relevant solution and their ligand interactions.

Keywords

G-quadruplex structures G-quadruplex-interactive compounds NMR spectroscopy

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Grants

  1. P30 CA023168/NCI NIH HHS
  2. R01 CA122952/NCI NIH HHS
  3. R01 CA177585/NCI NIH HHS

MeSH Term

G-Quadruplexes
Magnetic Resonance Imaging
Magnetic Resonance Spectroscopy
Nucleic Acid Conformation
RNA, Messenger

Chemicals

RNA, Messenger
Journal Article Research Support, N.I.H., Extramural

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