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Scientific reports
09 02, 2019;9 (1): 12625.

A novel magnet-based scratch method for standardisation of wound-healing assays.

M Fenu, T Bettermann, C Vogl, N Darwish-Miranda, J Schramel, F Jenner, I Ribitsch
Author Information
  1. M Fenu: University of Veterinary Medicine Vienna, Department of Companion Animals and Horses, Equine Surgery Unit, VETERM, Veterinaerplatz 1, 1210, Vienna, Austria.
  2. T Bettermann: University of Veterinary Medicine Vienna, Department of Companion Animals and Horses, Equine Surgery Unit, VETERM, Veterinaerplatz 1, 1210, Vienna, Austria.
  3. C Vogl: University of Veterinary Medicine Vienna, Department of Biomedical Sciences, Institute of Animal Breeding and Genetics, Veterinaerplatz 1, 1210, Vienna, Austria. ORCID
  4. N Darwish-Miranda: IST Austria, Bioimaging Facility, AM Campus 1, 3400, Klosterneuburg, Austria.
  5. J Schramel: University of Veterinary Medicine Vienna, Department of Companion Animals and Horses, Equine Surgery Unit, VETERM, Veterinaerplatz 1, 1210, Vienna, Austria.
  6. F Jenner: University of Veterinary Medicine Vienna, Department of Companion Animals and Horses, Equine Surgery Unit, VETERM, Veterinaerplatz 1, 1210, Vienna, Austria. Florien.Jenner@vetmeduni.ac.at. ORCID
  7. I Ribitsch: University of Veterinary Medicine Vienna, Department of Companion Animals and Horses, Equine Surgery Unit, VETERM, Veterinaerplatz 1, 1210, Vienna, Austria.
PMID: 31477739 DOI: 10.1038/s41598-019-48930-7

Abstract

A novel magnetic scratch method achieves repeatability, reproducibility and geometric control greater than pipette scratch assays and closely approximating the precision of cell exclusion assays while inducing the cell injury inherently necessary for wound healing assays. The magnetic scratch is affordable, easily implemented and standardisable and thus may contribute toward better comparability of data generated in different studies and laboratories.

References

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MeSH Term

Animals
Biological Assay
Cells, Cultured
Chondrocytes
Horses
Magnetic Phenomena
Reference Standards
Wound Healing
Journal Article

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