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Drug design, development and therapy
2019;13 2865-2871.

Validated UPLC-MS/MS method for quantification of fruquintinib in rat plasma and its application to pharmacokinetic study.

Yi-Bin Mei, Shun-Bin Luo, Ling-Yan Ye, Qiang Zhang, Jing Guo, Xiang-Jun Qiu, Sai-Li Xie
Author Information
  1. Yi-Bin Mei: Department of Cardiology, The People's Hospital of Lishui, Lishui, Zhejiang 323000, People's Republic of China.
  2. Shun-Bin Luo: Department of Clinical Pharmacy, The People's Hospital of Lishui, Lishui, Zhejiang 323000, People's Republic of China.
  3. Ling-Yan Ye: Department of Cardiology, The People's Hospital of Lishui, Lishui, Zhejiang 323000, People's Republic of China.
  4. Qiang Zhang: Department of Clinical Laboratory, The People's Hospital of Lishui, Lishui, Zhejiang 323000, People's Republic of China.
  5. Jing Guo: Department of Regional Medical Union, The People's Hospital of Lishui, Lishui, Zhejiang 323000, People's Republic of China.
  6. Xiang-Jun Qiu: Department of pharmacology, Medical College of Henan University of Science and Technology, Luoyang 471003, People's Republic of China.
  7. Sai-Li Xie: Department of Ultrasonic imaging, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325035, People's Republic of China.
PMID: 31616134 DOI: 10.2147/DDDT.S199362

Abstract

A new, simple, and sensitive ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for quantification of fruquintinib was established to assess the pharmacokinetics of fruquintinib in the rat. The internal standard working solution was added to the plasma sample for extraction before analysis. The Acquity UPLC BEH C18 chromatography column (2.1 mm ×50 mm, 1.7 μm) was used to separated analytes under gradient elution using acetonitrile and 0.1% formic acid as the mobile phase. Positive multiple reaction monitoring modes were chosen to detect fruquintinib and diazepam (IS). The precursor-to-product ion transitions were 394.2 → 363.2 for fruquintinib and m/z 285 → 154 for IS. The current method was linear over the concentration range of 1.0-1000 ng/mL for fruquintinib with a correlation coefficient of 0.9992 or better. The matrix effect of fruquintinib and IS was acceptable under the current method. The intra- and interday precision (RSD%) and accuracy (RE%) were within 11.9% and ±13.7%, respectively. The recovery, stability, and sensitivity were validated according to the United States Food and Drug Administration (FDA) regulations for bioanalytical method validation. The analytical method had been validated and applied to a pharmacokinetic study of fruquintinib in rat.

Keywords

UPLC-MS/MS fruquintinib pharmacokinetics rat plasma

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MeSH Term

Animals
Benzofurans
Chromatography, High Pressure Liquid
Molecular Structure
Quinazolines
Rats
Rats, Sprague-Dawley
Tandem Mass Spectrometry
Tissue Distribution

Chemicals

Benzofurans
Quinazolines
HMPL-013
Journal Article

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