Capillary electrophoresis for the investigation of two novel aminoalkanol derivatives of 1,7-diethyl-8,9-diphenyl-4-azatricyclo[5.2.1.02,6] dec-8-ene-3,5,10-trione as potential anticancer drugs in water solution and serum.

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Błażej Grodner, Mariola Napiórkowska

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Błażej Grodner: Chair and Department of Biochemistry and Pharmacogenomic, Medical University of Warsaw, Warsaw, Poland. ORCID
Mariola Napiórkowska: Chair and Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland.

PMID: 31705790 DOI: 10.1002/jssc.201900648

A simple, rapid, capillary zone electrophoresis method was developed and validated for the analysis of two novel aminoalkanol derivatives (I) and (II) of 1,7-diethyl-8,9-diphenyl-4-azatricyclo[5.2.1.0 ]dec-8-ene-3,5,10-trione, which were found in earlier studies as potential anticancer drugs. Samples were analyzed to demonstrate the specificity and stability indicating ability of the developed method. The samples were extracted using n-hexane-ethyl acetate mixture in the ratio of 90:10. Electrophoretic separation was performed on a eCAP fused silica capillary (37 cm length, 50 µm inside diameter) with a 50 mM tetraborate buffer as a background electrolyte adjusted to pH = 2.5. The separation time of (I) and (II) was achieved within 7 min. In addition, analysis of the two compounds in the serum was conducted. Limits of detection of (I) and (II) by UV absorbance at 200 nm were achieved in the range of 87.4-92.1 ng/mL. The sufficient recovery was observed in the range of 90.3-99.8%. The quantification limits for the compounds (I) and (II) were in the range of 279.71-291.03 ng/mL, respectively. The method has been successfully applied to the analysis of compounds (I) and (II) in serum samples.

anticancer drugs capillary electrophoresis drug monitoring

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/Medical University of Warsaw

Amino Alcohols

Antineoplastic Agents

Drug Stability

Electrophoresis, Capillary

Humans

Molecular Structure

Solutions

Water

Amino Alcohols

Antineoplastic Agents

Solutions

Water

Journal Article

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