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Nature communications
11 22, 2019;10 (1): 5303.

Glycerol-3-phosphate mediates rhizobia-induced systemic signaling in soybean.

M B Shine, Qing-Ming Gao, R V Chowda-Reddy, Asheesh K Singh, Pradeep Kachroo, Aardra Kachroo
Author Information
  1. M B Shine: Department of Plant Pathology, University of Kentucky, Lexington, KY, 40546, USA.
  2. Qing-Ming Gao: Department of Plant Pathology, University of Kentucky, Lexington, KY, 40546, USA.
  3. R V Chowda-Reddy: Department of Agronomy, Iowa State University, Ames, IA, 50011, USA.
  4. Asheesh K Singh: Department of Agronomy, Iowa State University, Ames, IA, 50011, USA. ORCID
  5. Pradeep Kachroo: Department of Plant Pathology, University of Kentucky, Lexington, KY, 40546, USA. ORCID
  6. Aardra Kachroo: Department of Plant Pathology, University of Kentucky, Lexington, KY, 40546, USA. apkach2@uky.edu.
PMID: 31757957 DOI: 10.1038/s41467-019-13318-8

Abstract

Glycerol-3-phosphate (G3P) is a well-known mobile regulator of systemic acquired resistance (SAR), which provides broad spectrum systemic immunity in response to localized foliar pathogenic infections. We show that G3P-derived foliar immunity is also activated in response to genetically-regulated incompatible interactions with nitrogen-fixing bacteria. Using gene knock-down we show that G3P is essential for strain-specific exclusion of non-desirable root-nodulating bacteria and the associated foliar pathogen immunity in soybean. Grafting studies show that while recognition of rhizobium incompatibility is root driven, bacterial exclusion requires G3P biosynthesis in the shoot. Biochemical analyses support shoot-to-root transport of G3P during incompatible rhizobia interaction. We describe a root-shoot-root signaling mechanism which simultaneously enables the plant to exclude non-desirable nitrogen-fixing rhizobia in the root and pathogenic microbes in the shoot.

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MeSH Term

Gene Knockdown Techniques
Glycerophosphates
Plant Immunity
Plant Proteins
Plant Roots
Plant Shoots
Rhizobium
Signal Transduction
Glycine max
Symbiosis

Chemicals

Glycerophosphates
Plant Proteins
alpha-glycerophosphoric acid
Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Links to CNCB-NGDC Resources

GEN: GEND000270 (RNA-seq analysis of distal gene expression changes during nodulation)

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