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01 21, 2020;10 (1): 897.

Transcriptional trajectories of anther development provide candidates for engineering male fertility in sorghum.

Namrata Dhaka, Kushagra Krishnan, Manu Kandpal, Ira Vashisht, Madan Pal, Manoj Kumar Sharma, Rita Sharma
Author Information
  1. Namrata Dhaka: Crop Genetics & Informatics Group, School of Computational and Integrative Sciences, Jawaharlal Nehru University, New Mehrauli Road, New Delhi, 110067, India.
  2. Kushagra Krishnan: Crop Genetics & Informatics Group, School of Computational and Integrative Sciences, Jawaharlal Nehru University, New Mehrauli Road, New Delhi, 110067, India.
  3. Manu Kandpal: Crop Genetics & Informatics Group, School of Computational and Integrative Sciences, Jawaharlal Nehru University, New Mehrauli Road, New Delhi, 110067, India.
  4. Ira Vashisht: Crop Genetics & Informatics Group, School of Computational and Integrative Sciences, Jawaharlal Nehru University, New Mehrauli Road, New Delhi, 110067, India.
  5. Madan Pal: Division of Plant Physiology, Indian Agricultural Research Institute, Pusa, New Delhi, 110012, India.
  6. Manoj Kumar Sharma: Crop Genetics & Informatics Group, School of Biotechnology, Jawaharlal Nehru University, New Mehrauli Road, New Delhi, 110067, India.
  7. Rita Sharma: Crop Genetics & Informatics Group, School of Computational and Integrative Sciences, Jawaharlal Nehru University, New Mehrauli Road, New Delhi, 110067, India. rita.genomics@gmail.com.
PMID: 31964983 DOI: 10.1038/s41598-020-57717-0

Abstract

Sorghum is a self-pollinated crop with multiple economic uses as cereal, forage, and biofuel feedstock. Hybrid breeding is a cornerstone for sorghum improvement strategies that currently relies on cytoplasmic male sterile lines. To engineer genic male sterility, it is imperative to examine the genetic components regulating anther/pollen development in sorghum. To this end, we have performed transcriptomic analysis from three temporal stages of developing anthers that correspond to meiotic, microspore and mature pollen stages. A total of 5286 genes were differentially regulated among the three anther stages with 890 of them exhibiting anther-preferential expression. Differentially expressed genes could be clubbed into seven distinct developmental trajectories using K-means clustering. Pathway mapping revealed that genes involved in cell cycle, DNA repair, regulation of transcription, brassinosteroid and auxin biosynthesis/signalling exhibit peak expression in meiotic anthers, while those regulating abiotic stress, carbohydrate metabolism, and transport were enriched in microspore stage. Conversely, genes associated with protein degradation, post-translational modifications, cell wall biosynthesis/modifications, abscisic acid, ethylene, cytokinin and jasmonic acid biosynthesis/signalling were highly expressed in mature pollen stage. High concurrence in transcriptional dynamics and cis-regulatory elements of differentially expressed genes in rice and sorghum confirmed conserved developmental pathways regulating anther development across species. Comprehensive literature survey in conjunction with orthology analysis and anther-preferential accumulation enabled shortlisting of 21 prospective candidates for in-depth characterization and engineering male fertility in sorghum.

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MeSH Term

Carbohydrate Metabolism
Cell Wall
Flowers
Gene Expression Regulation, Plant
Genetic Engineering
Genomics
Meiosis
Oryza
Plant Growth Regulators
Plant Infertility
Plant Proteins
Plants, Genetically Modified
Pollen
Reproducibility of Results
Secondary Metabolism
Sequence Analysis, RNA
Sorghum

Chemicals

Plant Growth Regulators
Plant Proteins
Journal Article Research Support, Non-U.S. Gov't

Links to CNCB-NGDC Resources

GEN: GEND000278 (Transcriptional trajectories of anther development provide candidates for engineering male fertility in sorghum.)

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