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Nature communications
09 08, 2020;11 (1): 4483.

Single-cell transcriptome maps of myeloid blood cell lineages in Drosophila.

Bumsik Cho, Sang-Ho Yoon, Daewon Lee, Ferdinand Koranteng, Sudhir Gopal Tattikota, Nuri Cha, Mingyu Shin, Hobin Do, Yanhui Hu, Sue Young Oh, Daehan Lee, A Vipin Menon, Seok Jun Moon, Norbert Perrimon, Jin-Wu Nam, Jiwon Shim
Author Information
  1. Bumsik Cho: Department of Life Sciences, College of Natural Science, Hanyang University, Seoul, 04736, Republic of Korea.
  2. Sang-Ho Yoon: Department of Life Sciences, College of Natural Science, Hanyang University, Seoul, 04736, Republic of Korea. ORCID
  3. Daewon Lee: Department of Life Sciences, College of Natural Science, Hanyang University, Seoul, 04736, Republic of Korea.
  4. Ferdinand Koranteng: Department of Life Sciences, College of Natural Science, Hanyang University, Seoul, 04736, Republic of Korea.
  5. Sudhir Gopal Tattikota: Department of Genetics, Harvard Medical School, Boston, MA, 02115, USA. ORCID
  6. Nuri Cha: Department of Life Sciences, College of Natural Science, Hanyang University, Seoul, 04736, Republic of Korea.
  7. Mingyu Shin: Department of Life Sciences, College of Natural Science, Hanyang University, Seoul, 04736, Republic of Korea.
  8. Hobin Do: Department of Life Sciences, College of Natural Science, Hanyang University, Seoul, 04736, Republic of Korea. ORCID
  9. Yanhui Hu: Department of Genetics, Harvard Medical School, Boston, MA, 02115, USA.
  10. Sue Young Oh: Department of Oral Biology, Yonsei University, College of Dentistry, Seoul, 03722, Republic of Korea.
  11. Daehan Lee: Department of Life Sciences, College of Natural Science, Hanyang University, Seoul, 04736, Republic of Korea.
  12. A Vipin Menon: Department of Life Sciences, College of Natural Science, Hanyang University, Seoul, 04736, Republic of Korea. ORCID
  13. Seok Jun Moon: Department of Oral Biology, Yonsei University, College of Dentistry, Seoul, 03722, Republic of Korea. ORCID
  14. Norbert Perrimon: Department of Genetics, Harvard Medical School, Boston, MA, 02115, USA.
  15. Jin-Wu Nam: Department of Life Sciences, College of Natural Science, Hanyang University, Seoul, 04736, Republic of Korea. jwnam@hanyang.ac.kr. ORCID
  16. Jiwon Shim: Department of Life Sciences, College of Natural Science, Hanyang University, Seoul, 04736, Republic of Korea. jshim@hanyang.ac.kr. ORCID
PMID: 32900993 DOI: 10.1038/s41467-020-18135-y

Abstract

The Drosophila lymph gland, the larval hematopoietic organ comprised of prohemocytes and mature hemocytes, has been a valuable model for understanding mechanisms underlying hematopoiesis and immunity. Three types of mature hemocytes have been characterized in the lymph gland: plasmatocytes, lamellocytes, and crystal cells, which are analogous to vertebrate myeloid cells, yet molecular underpinnings of the lymph gland hemocytes have been less investigated. Here, we use single-cell RNA sequencing to comprehensively analyze heterogeneity of developing hemocytes in the lymph gland, and discover previously undescribed hemocyte types including adipohemocytes, stem-like prohemocytes, and intermediate prohemocytes. Additionally, we identify the developmental trajectory of hemocytes during normal development as well as the emergence of the lamellocyte lineage following active cellular immunity caused by wasp infestation. Finally, we establish similarities and differences between embryonically derived- and larval lymph gland hemocytes. Altogether, our study provides detailed insights into the hemocyte development and cellular immune responses at single-cell resolution.

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Grants

  1. /Howard Hughes Medical Institute

MeSH Term

Animals
Animals, Genetically Modified
Cell Differentiation
Cell Lineage
Drosophila melanogaster
Ectoparasitic Infestations
Gene Expression Profiling
Hematopoiesis
Hemocytes
Host-Parasite Interactions
Lymphoid Tissue
RNA-Seq
Single-Cell Analysis
Transcriptome
Wasps
Journal Article Research Support, Non-U.S. Gov't

Links to CNCB-NGDC Resources

GEN: GEND000148 (A single cell transcriptome atlas of myeloid blood cell lineages in Drosophila [scRNA-seq])

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