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Jan, 2021;18 (1): e2000829.

β-Carboline-Based pH Fluorescent Probe and Its Application for Monitoring Enzymatic Ester Hydrolysis.

Rong Huang, Xian-Dong Zhang, Xing Wu, Ji-Kai Liu
Author Information
  1. Rong Huang: School of Pharmaceutical Sciences, South-Central University of Nationalities, Wuhan, 430074, P. R. China. ORCID
  2. Xian-Dong Zhang: School of Pharmaceutical Sciences, South-Central University of Nationalities, Wuhan, 430074, P. R. China.
  3. Xing Wu: School of Pharmaceutical Sciences, South-Central University of Nationalities, Wuhan, 430074, P. R. China.
  4. Ji-Kai Liu: School of Pharmaceutical Sciences, South-Central University of Nationalities, Wuhan, 430074, P. R. China.
PMID: 33188535 DOI: 10.1002/cbdv.202000829

Abstract

A novel pH-activatable fluorescent probe, 1-(propan-2-yl)-9H-pyrido[3,4-b]indole-3-carboxylic acid (L-1), based on β-carboline derivatives, has been developed, which displays significant fluorescent response toward pH variation with high selectivity, good photo-stability and favorable pKa value. Moreover, L-1 can dynamically monitor the release of protons during ester hydrolysis reaction in consistent with enzymatic kinetics manner.

Keywords

enzymatic ester hydrolysis pH probe β-carboline

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Grants

  1. 21502239/National Natural Science Foundation of China
  2. /Fundamental Research Funds for the Central Universities
  3. CZQ19013/South-Central University for Nationalities

MeSH Term

Carbolines
Carboxylesterase
Esters
Fluorescent Dyes
Hydrogen-Ion Concentration
Hydrolysis
Indoles
Kinetics
Metals

Chemicals

Carbolines
Esters
Fluorescent Dyes
Indoles
Metals
indole-3-carboxylic acid
Carboxylesterase
Journal Article

Word Cloud

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