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May, 2021;6 (5): 1267-1282.

A thermostability perspective on enhancing physicochemical and cytological characteristics of octacalcium phosphate by doping iron and strontium.

Haishan Shi, Xiaoling Ye, Jing Zhang, Tingting Wu, Tao Yu, Changren Zhou, Jiandong Ye
Author Information
  1. Haishan Shi: School of Stomatology, Jinan University, Guangzhou, 510632, China.
  2. Xiaoling Ye: National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, 510006, China.
  3. Jing Zhang: National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, 510006, China.
  4. Tingting Wu: National Engineering Research Center for Healthcare Devices, Guangdong Institute of Medical Instruments, Guangdong Academy of Sciences, Guangzhou, 510500, China.
  5. Tao Yu: College of Chemistry and Materials Science, Jinan University, Guangzhou, 510632, China.
  6. Changren Zhou: College of Chemistry and Materials Science, Jinan University, Guangzhou, 510632, China.
  7. Jiandong Ye: National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, 510006, China.
PMID: 33210024 DOI: 10.1016/j.bioactmat.2020.10.025

Abstract

Investigation of thermostability will lead the groundbreaking of unraveling the mechanism of influence of ion-doping on the properties of calcium phosphates. In this work, octacalcium phosphate (OCP), a metastable precursor of biological apatite, was used as a stability model for doping ions (Fe and Sr) with different ionic charges and radii. After treated under hot air at different temperatures (110-200 °C), the phase, morphology, structure, physicochemical properties, protein affinity, ions release, and cytological responses of the ion-doped OCPs were investigated comparatively. The results showed that the collapse of OCP crystals gradually occurred, accompanying with the dehydration of hydrated layers and the disintegration of plate-like crystals as the temperature increased. The collapsed crystals still retained the typical properties of OCP and the potential of conversion into hydroxyapatite. Compared to the undoped OCP, Fe-OCP, and Sr-OCP had lower and higher thermostability respectively, leading to different material surface properties and ions release. The adjusted thermostability of Fe-OCP and Sr-OCP significantly enhanced the adsorption of proteins (BSA and LSZ) and the cytological behavior (adhesion, spreading, proliferation, and osteogenic differentiation) of bone marrow mesenchymal stem cells to a varying extent under the synergistic effects of corresponding surface characteristics and early active ions release. This work paves the way for understanding the modification mechanism of calcium phosphates utilizing ion doping strategy and developing bioactive OCP-based materials for tissue repair.

Keywords

Iron Octacalcium phosphate Osteogenesis Strontium Thermostability

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