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Reproduction in domestic animals = Zuchthygiene
May, 2021;56 (5): 801-811.

scRNA-seq of ovarian follicle granulosa cells from different fertility goats reveals distinct expression patterns.

Zengkuan Li, Junjie Wang, Yong Zhao, Dongxue Ma, Minghui Zhao, Na Li, Yuhao Men, Yuan Zhang, Huimin Chu, Chuzhao Lei, Wei Shen, Othman El-Mahdy Othman, Lingjiang Min
Author Information
  1. Zengkuan Li: College of Animal Sciences and Technology, Qingdao Agricultural University, Qingdao, China.
  2. Junjie Wang: College of Life Sciences, Qingdao Agricultural University, Qingdao, China.
  3. Yong Zhao: College of Life Sciences, Qingdao Agricultural University, Qingdao, China. ORCID
  4. Dongxue Ma: College of Animal Sciences and Technology, Qingdao Agricultural University, Qingdao, China.
  5. Minghui Zhao: College of Life Sciences, Qingdao Agricultural University, Qingdao, China.
  6. Na Li: College of Life Sciences, Qingdao Agricultural University, Qingdao, China.
  7. Yuhao Men: College of Animal Sciences and Technology, Qingdao Agricultural University, Qingdao, China.
  8. Yuan Zhang: Jining Animal Husbandry Development Center, Jining, China.
  9. Huimin Chu: Jining Agricultural Science Institute, Jining, China.
  10. Chuzhao Lei: Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.
  11. Wei Shen: College of Life Sciences, Qingdao Agricultural University, Qingdao, China.
  12. Othman El-Mahdy Othman: Cell Biology Department, National Research Centre, Giza, Egypt.
  13. Lingjiang Min: College of Animal Sciences and Technology, Qingdao Agricultural University, Qingdao, China.
PMID: 33624340 DOI: 10.1111/rda.13920

Abstract

The new technology of high-throughput single-cell RNA sequencing (10 × scRNA-seq) was developed recently with many advantages. However, it was not commonly used in farm animal research. There are few reports for the gene expression of goat ovarian follicle granulosa cells (GCs) during different developmental stages. In the current investigation, the gene expression of follicle GCs at different stages from two populations of Ji'ning grey goats: high litter size (HL; ≥3/L; 2 L) and low litter size (LL; ≤2 /L; 2 L) were analysed by scRNA-seq. Many GC marker genes were identified, and the pseudo-time showed that GCs developed during the time course which reflected the follicular development and differentiation trajectory. Moreover, the gene expression difference between the two populations HL versus LL was very clear at different developmental stages. Many marker genes differentially expressed at different developmental stages. ASIP and ASPN were found to be highly expressed in the early stage of GCs, INHA, INHBA, MFGE8 and HSD17B1 were identified to be highly expressed in the growing stage of GCs, while IGFBP2, IGFBP5 and CYP11A1 were found to be highly expressed in late stage. These marker genes could be used as reference genes of goat follicle GC development. This investigation for the first time discovered the gene expression patterns in goat follicle GCs in high- or low-fertility populations (based on litter size) by scRNA-seq which may be useful for uncovering the oocyte development potential.

Keywords

goat granulosa cells litter size scRNA-seq

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Grants

  1. 2017YFE0113600/National Key Research and Development Program of China
  2. 31772569/National Natural Science Foundation of China
  3. SDAIT-10-08/Technology System of Modern Agricultural Industry in Shandong Province

MeSH Term

Animals
Female
Fertility
Gene Expression Profiling
Goats
Granulosa Cells
Litter Size
Ovarian Follicle
RNA, Small Cytoplasmic

Chemicals

RNA, Small Cytoplasmic
Journal Article

Links to CNCB-NGDC Resources

GEN: GEND000066 (Single-cell RNA-seq of the goat ovarian granulosa cells samples from high and low fertility groups)
GEN: GEND000566 (Single-cell RNA-seq (10x) of the goat ovarian granulosa cells samples from high fertility group (>three babys per birth) and low fertility group (< two babys per birth).)

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